FoxO1, also called FKHR (forkhead in rhabdomyosarcoma), is a ubiquitously expressed member of the forkhead box O family of winged helix transcription factors. In neurons, it is activated in response to stress, translocating to the nucleus where it promotes apoptosis and blocks proliferation. In insulin-responsive tissues, nutrient abundance triggers phosphorylation by AKT that blocks nuclear translocation and activity.
FoxO1/FKHR Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-24857
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Bovine (98%), Mouse (96%), Porcine (98%), Rat (96%). Backed by our 100% Guarantee.
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
A portion of amino acids 250-300 of human FOXO1A protein was used as the immunogen.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus (86%), Zebrafish (80%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for FoxO1/FKHR Antibody - BSA Free
Western Blot: FoxO1/FKHR AntibodyBSA Free [NBP2-24857]
Western Blot: FoxO1/FKHR Antibody [NBP2-24857] - Analysis of FOXO1A in human ovary lysate in the (1) absence and (2) presence of immunizing peptide using this antibody at 0.05 ug/ml.Applications for FoxO1/FKHR Antibody - BSA Free
Application
Recommended Usage
Western Blot
0.05-0.50 ug/ml
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: FoxO1/FKHR
Additional FoxO1/FKHR Products
Product Documents for FoxO1/FKHR Antibody - BSA Free
Certificate of Analysis
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Product Specific Notices for FoxO1/FKHR Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
IL-4 Signaling Pathways
IL-7 Signaling Pathways
IL-9 Signaling Pathways
IL-15 Signaling Pathways
IL-21 Signaling Pathways
MAPK Signaling: Oxidative Stress Pathway
TGF-beta Signaling Pathways
VEGF - VEGF R2 Signaling Pathways