FOXO3 [p Ser253] Antibody (1E1) - BSA Free
Novus Biologicals | Catalog # NBP3-26424
Recombinant Monoclonal Antibody
![FOXO3 [p Ser253] Antibody (1E1)](https://resources.rndsystems.com/images/products/nbp3-26424_rabbit-foxo3-p-ser-253-mab-1e1-262202415355710.jpg "Immunohistochemistry: FOXO3 [p Ser253] Antibody (1E1) [NBP3-26424] -")
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot, ELISA
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1E1 expressed in HEK293
Format
BSA Free
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Product Specifications
Immunogen
A synthesized peptide derived from Human FOXO3 [p Ser253] [UniProt O43524]
Modification
p Ser 253
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for FOXO3 [p Ser253] Antibody (1E1) - BSA Free
Immunohistochemistry: FOXO3 [p Ser253] Antibody (1E1) [NBP3-26424] -
Immunohistochemistry: FOXO3 [p Ser253] Antibody (1E1) [NBP3-26424] - Image of FOXO3 [p Ser253] Antibody (1E1) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.![FOXO3 [p Ser253] Antibody (1E1)](https://resources.rndsystems.com/images/products/nbp3-26424_rabbit-foxo3-p-ser-253-mab-1e1-2722024857334.jpg "Immunohistochemistry: FOXO3 [p Ser253] Antibody (1E1) [NBP3-26424] -")
Immunohistochemistry: FOXO3 [p Ser253] Antibody (1E1) [NBP3-26424] -
Immunohistochemistry: FOXO3 [p Ser253] Antibody (1E1) [NBP3-26424] - Image of FOXO3 [p Ser253] Antibody (1E1) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.![FOXO3 [p Ser253] Antibody (1E1)](https://resources.rndsystems.com/images/products/nbp3-26424_rabbit-foxo3-p-ser-253-mab-1e1-282202411442618.jpg "Western Blot: FOXO3 [p Ser253] Antibody (1E1) [NBP3-26424] -")
Western Blot: FOXO3 [p Ser253] Antibody (1E1) [NBP3-26424] -
Western Blot: FOXO3 [p Ser253] Antibody (1E1) [NBP3-26424] - Positive Western Blot detected in Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate.All lanes: FOXO3 [p Ser253] Antibody at 0.96ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 97 KDa
Observed band size: 97 KDa
Applications for FOXO3 [p Ser253] Antibody (1E1) - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl, and 50% glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: FoxO3
Long Name
Forkhead Box O3
Alternate Names
AF6q21, FKHRL1, FKHRL1P2, FOXO3A
Gene Symbol
FOXO3
Additional FoxO3 Products
Product Documents for FOXO3 [p Ser253] Antibody (1E1) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for FOXO3 [p Ser253] Antibody (1E1) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
Common Cytokine Receptor Gamma-Chain Family Signaling Pathways
IL-2 Signaling Pathways
IL-4 Signaling Pathways
IL-7 Signaling Pathways
IL-9 Signaling Pathways
IL-15 Signaling Pathways
IL-21 Signaling Pathways
MAPK Signaling: Oxidative Stress Pathway
TGF-beta Signaling Pathways
IL-15 Signaling Pathways
IL-21 Signaling Pathways
MAPK Signaling: Oxidative Stress Pathway
TGF-beta Signaling Pathways