FOXO3 [p Ser425] Antibody - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-16945
![Western Blot: FOXO3 [p Ser425] AntibodyAzide and BSA Free [NBP3-16945]](https://resources.rndsystems.com/images/products/FOXO3-[p-Ser425]-Antibody-Western-Blot-NBP3-16945-img0004.jpg "Western Blot: FOXO3 [p Ser425] AntibodyAzide and BSA Free [NBP3-16945]")
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Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
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Product Specifications
Immunogen
A synthetic phosphorylated peptide around S425 of human FOXO3 (NP_001446.1). LGSPT
Modification
p Ser 425
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for FOXO3 [p Ser425] Antibody - Azide and BSA Free
Western Blot: FOXO3 [p Ser425] AntibodyAzide and BSA Free [NBP3-16945]
Western Blot: FOXO3 [p Ser425] Antibody [NBP3-16945] - Western blot analysis of extracts of K-562 cells, using FOXO3 antibody (NBP3-16945) at 1:1000 dilution.K-562 cells were treated by 10% FBS at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.![Immunohistochemistry-Paraffin: FOXO3 [p Ser425] Antibody - Azide and BSA Free [NBP3-16945]](https://resources.rndsystems.com/images/products/FOXO3-[p-Ser425]-Antibody-Immunohistochemistry-Paraffin-NBP3-16945-img0003.jpg "Immunohistochemistry-Paraffin: FOXO3 [p Ser425] Antibody - Azide and BSA Free [NBP3-16945]")
Immunohistochemistry-Paraffin: FOXO3 [p Ser425] Antibody - Azide and BSA Free [NBP3-16945]
Immunohistochemistry-Paraffin: FOXO3 [p Ser425] Antibody [NBP3-16945] - Immunohistochemistry of paraffin-embedded mouse heart using FOXO3 Rabbit pAb (NBP3-16945) at dilution of 1:250 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.![Immunohistochemistry-Paraffin: FOXO3 [p Ser425] Antibody - Azide and BSA Free [NBP3-16945]](https://resources.rndsystems.com/images/products/FOXO3-[p-Ser425]-Antibody-Immunohistochemistry-Paraffin-NBP3-16945-img0001.jpg "Immunohistochemistry-Paraffin: FOXO3 [p Ser425] Antibody - Azide and BSA Free [NBP3-16945]")
Immunohistochemistry-Paraffin: FOXO3 [p Ser425] Antibody - Azide and BSA Free [NBP3-16945]
Immunohistochemistry-Paraffin: FOXO3 [p Ser425] Antibody [NBP3-16945] - Immunohistochemistry of paraffin-embedded rat kidney using FOXO3 Rabbit pAb (NBP3-16945) at dilution of 1:250 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.![Immunohistochemistry-Paraffin: FOXO3 [p Ser425] Antibody - Azide and BSA Free [NBP3-16945]](https://resources.rndsystems.com/images/products/FOXO3-[p-Ser425]-Antibody-Immunohistochemistry-Paraffin-NBP3-16945-img0002.jpg "Immunohistochemistry-Paraffin: FOXO3 [p Ser425] Antibody - Azide and BSA Free [NBP3-16945]")
Immunohistochemistry-Paraffin: FOXO3 [p Ser425] Antibody - Azide and BSA Free [NBP3-16945]
Immunohistochemistry-Paraffin: FOXO3 [p Ser425] Antibody [NBP3-16945] - Immunohistochemistry of paraffin-embedded human lung cancer using FOXO3 Rabbit pAb (NBP3-16945) at dilution of 1:250 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.Applications for FOXO3 [p Ser425] Antibody - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
Azide and BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: FoxO3
Long Name
Forkhead Box O3
Alternate Names
AF6q21, FKHRL1, FKHRL1P2, FOXO3A
Gene Symbol
FOXO3
Additional FoxO3 Products
Product Documents for FOXO3 [p Ser425] Antibody - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for FOXO3 [p Ser425] Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.govCustomer Reviews for FOXO3 [p Ser425] Antibody - Azide and BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
Common Cytokine Receptor Gamma-Chain Family Signaling Pathways
IL-2 Signaling Pathways
IL-4 Signaling Pathways
IL-7 Signaling Pathways
IL-9 Signaling Pathways
IL-15 Signaling Pathways
IL-21 Signaling Pathways
MAPK Signaling: Oxidative Stress Pathway
TGF-beta Signaling Pathways
IL-15 Signaling Pathways
IL-21 Signaling Pathways
MAPK Signaling: Oxidative Stress Pathway
TGF-beta Signaling Pathways