FoxP3 Antibody (BLR034F)

Novus Biologicals | Catalog # NBP3-14670

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Multiplex Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit Clone # BLR034F
View all FoxP3 Primary Antibodies »

Product Specifications

Immunogen

between 275 and 325

Clonality

Monoclonal

Host

Rabbit

Scientific Data Images for FoxP3 Antibody (BLR034F)

Western Blot: FoxP3 Antibody (BLR034F) [NBP3-14670]

Western Blot: FoxP3 Antibody (BLR034F) [NBP3-14670]

Western Blot: FoxP3 Antibody (BLR034F) [NBP3-14670] - Whole cell lysate (50 ug) from Jurkat, HEK293T, MJ, MOLT-4, and RPMI-8226 cells prepared using NETN lysis buffer. Rabbit anti-FOXP3 recombinant monoclonal antibody (NBP3-14670 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (NB7156). Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody.
Immunohistochemistry: FoxP3 Antibody (BLR034F) [NBP3-14670]

Immunohistochemistry: FoxP3 Antibody (BLR034F) [NBP3-14670]

Immunohistochemistry: FoxP3 Antibody (BLR034F) [NBP3-14670] - FFPE section of human breast carcinoma. Antibody: Rabbit anti-FOXP3recombinant monoclonal antibody used at 1:250. Secondary: HRP-conjugated goat antirabbit IgG. Substrate: Opal™. Counterstain: DAPI (blue).
Immunohistochemistry-Paraffin: FoxP3 Antibody (BLR034F) [NBP3-14670]

Immunohistochemistry-Paraffin: FoxP3 Antibody (BLR034F) [NBP3-14670]

Immunohistochemistry-Paraffin: FoxP3 Antibody (BLR034F) [NBP3-14670] - Antibody: Rabbit anti-FOXP3 recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Immunohistochemistry-Paraffin: FoxP3 Antibody (BLR034F) [NBP3-14670]

Immunohistochemistry-Paraffin: FoxP3 Antibody (BLR034F) [NBP3-14670]

Immunohistochemistry-Paraffin: FoxP3 Antibody (BLR034F) [NBP3-14670] - Antibody: Rabbit anti-FOXP3 recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Immunohistochemistry: FoxP3 Antibody (BLR034F) [NBP3-14670]

Immunohistochemistry: FoxP3 Antibody (BLR034F) [NBP3-14670]

Immunohistochemistry: FoxP3 Antibody (BLR034F) [NBP3-14670] - Antibody: Rabbit anti-FOXP3 recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Immunoprecipitation: FoxP3 Antibody (BLR034F) [NBP3-14670]

Immunoprecipitation: FoxP3 Antibody (BLR034F) [NBP3-14670]

Immunoprecipitation: FoxP3 Antibody (BLR034F) [NBP3-14670] - Samples: Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from MJ cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-FOXP3 recombinant monoclonal antibody used for IP at 20 ul/mg lysate. FOXP3 was also immunoprecipitated by a previous lot of this antibody. For blotting immunoprecipitated FOXP3, was used at 1:1000. Chemiluminescence with an exposure time of 30 seconds.

Applications for FoxP3 Antibody (BLR034F)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 - 1:500

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100 - 1:500

Immunoprecipitation

20 ul/1mg lysate

Multiplex Immunofluorescence

1:250

Western Blot

1:1000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.

Formulation, Preparation, and Storage

Purification

>95%

Formulation

Borate Buffered Saline (BBS) pH 8.2 with, BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: FoxP3

Forkhead box P3 (FoxP3), aka Scurfin and JM2, is a protein involving in immune system responses and functioning as a transcriptional regulator crucial in the development and inhibitory of regulatory T-Cells (Treg). FoxP3 is a master regulator of the Treg lineage, who requires Ahr and STAT-5 and other additional transcription factors to retains its complete function and differentiation. Scurfin remains steady and constitutively expressed in two conditions: a high level in CD25 + CD4 positive regulatory T cells and a low level in CD4 positive/CD25 negative cells. JM2 is absent in CD4 negative/CD8 positive T cells. FoxP3 defects are related to various cancers and autoimmune diseases, and X-linked syndrome (IPEX), while overexpression may also cause severe immunodeficiency.

Long Name

Forkhead Box P3

Alternate Names

AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID

Gene Symbol

FOXP3

Additional FoxP3 Products

Product Documents for FoxP3 Antibody (BLR034F)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for FoxP3 Antibody (BLR034F)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for FoxP3 Antibody (BLR034F)

Showing  1 - 2 of 2 FAQs Showing All

  • Q: Does FoxP3 antibodies comes in lyophilized form?

    A: FoxP3 antibodies, aka JM2 IPEX, and Scurfin antibodies, have the following products in lyophilized form: NBP1-18319, AF3240.

  • Q: What the theoretical molecular weight for FoxP3 antibodies?

    A: The TMW of FoxP3 antibodies is approximately 47 - 52 kDa.

  • Q: Does FoxP3 antibodies comes in lyophilized form?

    A: FoxP3 antibodies, aka JM2 IPEX, and Scurfin antibodies, have the following products in lyophilized form: NBP1-18319, AF3240.

  • Q: What the theoretical molecular weight for FoxP3 antibodies?

    A: The TMW of FoxP3 antibodies is approximately 47 - 52 kDa.

Showing  1 - 2 of 2 FAQs Showing All
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