FoxP3 Antibody

Novus Biologicals | Catalog # NB600-243

Novus Biologicals
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Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all FoxP3 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence KRSQRPNKCSNP corresponding to C-Terminus according to Uniprot Mouse NP_473380.1.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Rat.

Localization

Nuclear

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Novus Biologicals Goat FoxP3 Antibody (NB600-243) is a polyclonal antibody validated for use in IHC, ELISA, Flow and ICC/IF. Anti-FoxP3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for FoxP3 Antibody

FoxP3 Antibody

Immunohistochemistry-Paraffin: FoxP3 Antibody [NB600-243] -

Immunohistochemistry-Paraffin: FoxP3 Antibody [NB600-243] - (5ug/ml) staining of paraffin embedded Mouse Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Flow Cytometry: FoxP3 Antibody [NB600-243]

Flow Cytometry: FoxP3 Antibody [NB600-243]

Flow Cytometry: FoxP3 Antibody [NB600-243] - Analysis of paraformaldehyde fixed NIH3T3 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunohistochemistry-Paraffin: FoxP3 Antibody [NB600-243]

Immunohistochemistry-Paraffin: FoxP3 Antibody [NB600-243]

Immunohistochemistry-Paraffin: FoxP3 Antibody [NB600-243] - Negative Control showing staining of paraffin embedded Mouse Spleen, with no primary antibody.

Applications for FoxP3 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunohistochemistry

2 - 4 ug/mL

Immunohistochemistry-Paraffin

2 - 4 ug/mL

Peptide ELISA

Detection limit 1:4000
Application Notes
IHC-P: Mouse thymus shows nuclear staining in some patches of thymocytes.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FoxP3

Forkhead box P3 (FoxP3), aka Scurfin and JM2, is a protein involving in immune system responses and functioning as a transcriptional regulator crucial in the development and inhibitory of regulatory T-Cells (Treg). FoxP3 is a master regulator of the Treg lineage, who requires Ahr and STAT-5 and other additional transcription factors to retains its complete function and differentiation. Scurfin remains steady and constitutively expressed in two conditions: a high level in CD25 + CD4 positive regulatory T cells and a low level in CD4 positive/CD25 negative cells. JM2 is absent in CD4 negative/CD8 positive T cells. FoxP3 defects are related to various cancers and autoimmune diseases, and X-linked syndrome (IPEX), while overexpression may also cause severe immunodeficiency.

Long Name

Forkhead Box P3

Alternate Names

AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID

Entrez Gene IDs

50943 (Human); 20371 (Mouse); 317382 (Rat)

Gene Symbol

FOXP3

UniProt

NP_473380.1 (Mouse)

Additional FoxP3 Products

Product Documents for FoxP3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for FoxP3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for FoxP3 Antibody

Showing  1 - 3 of 3 FAQs Showing All

    • A: FoxP3 antibodies, aka JM2 IPEX, and Scurfin antibodies, have the following products in lyophilized form: NBP1-18319, AF3240.

    • A: A sequence within the C-terminal region (KRSQRPNKCSNP) of FoxP3.

    • A: The TMW of FoxP3 antibodies is approximately 47 - 52 kDa.
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