Frizzled-5 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-02414
![Immunohistochemistry-Paraffin: Frizzled-5 Antibody - BSA Free [NBP1-02414]](https://resources.rndsystems.com/images/products/Frizzled-5-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP1-02414-img0002.jpg "Immunohistochemistry-Paraffin: Frizzled-5 Antibody - BSA Free [NBP1-02414]")
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Key Product Details
Species Reactivity
Validated:
Human, Porcine, Bovine, Canine, Monkey, Primate, Rabbit
Predicted:
Mouse (93%), Rat (93%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, ELISA
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Synthetic 14 amino acid peptide from N-terminal extracellular domain of human Frizzled-5.
Epitope
N-Terminus extracellular domain
Reactivity Notes
Gorilla, Gibbon, Marmoset. Predicted cross-reactivity based on sequence identity: Elephant (100%), Opossum (86%).
Specificity
Human Frizzled-5. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Product can be stored undiluted at 4C for up to 1 month.
Scientific Data Images for Frizzled-5 Antibody - BSA Free
Immunohistochemistry-Paraffin: Frizzled-5 Antibody - BSA Free [NBP1-02414]
Immunohistochemistry-Paraffin: Frizzled-5 Antibody - BSA Free [NBP1-02414] - Staining of human pancreas tissue after heat-induced antigen retrieval. Antibody concentration 7 ug/ml.Applications for Frizzled-5 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
7 ug/ml
Immunohistochemistry-Paraffin
7 ug/ml
Application Notes
.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Background: Frizzled-5
Additional Frizzled-5 Products
Product Documents for Frizzled-5 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Frizzled-5 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
Wnt Signaling Pathways: beta-Catenin-dependent Wnt Signaling
