FSTL5 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-04852

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all Follistatin-like 5/FSTL5 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 21-250 of human FSTL5 (NP_064501.2). RPTKEGGYGLKSYQPLMRLRHKQEKNQESSRVKGFMIQDGPFGSCENKYCGLGRHCVTSRETGQAECACMDLCKRHYKPVCGSDGEFYENHCEVHRAACLKKQKITIVHNEDCFFKGDKCKTTEYSKMKNMLLDLQNQKYIMQENENPNGDDISRKKLLVDQMFKYFDADSNGLVDINELTQVIKQEELGKDLFDCTLYVLLKYDDFNADKHLALEEFYRAFQVIQLSLP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

96 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FSTL5 Antibody - Azide and BSA Free

Western Blot: FSTL5 AntibodyAzide and BSA Free [NBP3-04852]

Western Blot: FSTL5 AntibodyAzide and BSA Free [NBP3-04852]

Western Blot: FSTL5 Antibody [NBP3-04852] - Analysis of extracts of various cell lines, using FSTL5 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit
FSTL5 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: FSTL5 Antibody - Azide and BSA Free [NBP3-04852] -

Immunocytochemistry/ Immunofluorescence: FSTL5 Antibody - Azide and BSA Free [NBP3-04852] - Immunofluorescence analysis of paraffin-embedded mouse brain using FSTL5 Rabbit pAb (A16553) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for FSTL5 Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Follistatin-like 5/FSTL5

Follistatin-like 5 (FSTL5) is a secreted SPARC family protein that is expressed in the cartilage and tendon of developing digits and the shafts of long bones. In the brain it is expressed in the olfactory bulb, CA3 area of the hippocampus, and granule cell layer of the cerebellum. FSTL5 inhibits the proliferation and promotes the apoptosis of hepatocellular carcinoma cells. Its expression in medulloblastoma and downregulation in hepatocellular carcinoma are associated with poor prognosis.

Alternate Names

Follistatin like 5, FSL5, FSTL5

Gene Symbol

FSTL5

Additional Follistatin-like 5/FSTL5 Products

Product Documents for FSTL5 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for FSTL5 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Related Research Areas

Activin Family

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Protocols

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FAQs

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