Q: Do you have any antibodies against FTO, where you can indicate the sequence of the immunogen?

A: With regards to other FTO antibodies that we do list the peptide sequence for, the immunogen sequence for catalog number NB110-60935 can be found in the range of amino acids 400-505. Many of the exact immunogen sequences used for our products are proprietary and cannot be disclosed. In these instances we try to provide a reasonable amino acid range to help in your decision.

FTO Antibody (2Q9K10)

Novus Biologicals | Catalog # NBP3-16285

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2Q9K10 expressed in HEK293

View all FTO Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human FTO (Q9C0B1). MKRTPTAEEREREAKKLRLLEELEDTWLPYLTPKDDEFYQQWQLKYPKLILREASSVSEELHKEVQEAFLTLHKHGCLFRDLVRIQGKDLLTPVSRILIG

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FTO Antibody (2Q9K10)

Western Blot: FTO Antibody (2Q9K10) [NBP3-16285]

Western Blot: FTO Antibody (2Q9K10) [NBP3-16285] - Analysis of extracts of various cell lines, using FTO Rabbit mAb (NBP3-16285) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Immunocytochemistry/ Immunofluorescence: FTO Antibody (2Q9K10) [NBP3-16285]

Immunocytochemistry/Immunofluorescence: FTO Antibody (2Q9K10) [NBP3-16285] - Immunofluorescence analysis of U-2 OS cells using FTO Rabbit mAb (NBP3-16285) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunohistochemistry-Paraffin: FTO Antibody (2Q9K10) [NBP3-16285]

Immunohistochemistry-Paraffin: FTO Antibody (2Q9K10) [NBP3-16285] - Human bladder cancer using FTO Rabbit mAb (NBP3-16285) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunoprecipitation: FTO Antibody (2Q9K10) [NBP3-16285]

Immunoprecipitation: FTO Antibody (2Q9K10) [NBP3-16285] - Analysis of 300ug extracts of 293T cells using 3ug FTO antibody (NBP3-16285). Western blot was performed from the immunoprecipitate using FTO antibody (NBP3-16285) at a dilition of 1:500.

Immunocytochemistry/ Immunofluorescence: FTO Antibody (2Q9K10) [NBP3-16285] -

Immunocytochemistry/ Immunofluorescence: FTO Antibody (2Q9K10) [NBP3-16285] - Confocal imaging of U-2 OS cells using FTO Rabbit mAb. The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

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Applications for FTO Antibody (2Q9K10)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Immunoprecipitation

1:100 - 1:500

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FTO

Fat mass and obesity-associated protein (FTO) is a novel protein and a member of the non-heme dioxygenase (Fe(II)- and 2-oxoglutarate-dependent dioxygenases) superfamily. Though not much is yet known about FTO, it is thought that the protein may somehow modify the activity of genes involved in metabolism and fat storage, which in turn may influence a person's risk of obesity.

FTO antibodies are useful tools for lipid metabolism research and obesity studies.

Long Name

Fat Mass and Obesity Associated

Alternate Names

Alpha-ketoglutarate-dependent dioxygenase FTO

Gene Symbol

FTO

Additional FTO Products

Product Documents for FTO Antibody (2Q9K10)

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Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for FTO Antibody (2Q9K10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Diabetes

Obesity

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