Fumarase Antibody (7612) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-43578

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (98%), Chicken (91%), Porcine (98%), Primate (99%), Sheep (99%), Zebrafish (90%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Clone # 7612

Format

Azide and BSA Free
View all Fumarase Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Fumarase. The exact sequence is proprietary.

Reactivity Notes

Chimpanzee (99%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Fumarase Antibody (7612) - Azide and BSA Free

Western Blot: Fumarase Antibody (7612) [NBP2-43578]

Western Blot: Fumarase Antibody (7612) [NBP2-43578]

Western Blot: Fumarase Antibody (7612) [NBP2-43578] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Fumarate hydratase antibody [7612].
Western Blot: Fumarase Antibody (7612) [NBP2-43578]

Western Blot: Fumarase Antibody (7612) [NBP2-43578]

Western Blot: Fumarase Antibody (7612) [NBP2-43578] - Analysis of A. 30 ug 293T B. 30 ug A431 C. 30 ug HeLa D. 30 ug HepG2 10 % SDS-PAGE Fumarate hydratase antibody [7612] dilution: 1:500.
Western Blot: Fumarase Antibody (7612) [NBP2-43578]

Western Blot: Fumarase Antibody (7612) [NBP2-43578]

Western Blot: Fumarase Antibody (7612) [NBP2-43578] - Analysis of A. 50 ug mouse liver lysate/extract 10 % SDS-PAGE Fumarate hydratase antibody [7612] dilution: 1:1000
Western Blot: Fumarase Antibody (7612) [NBP2-43578]

Western Blot: Fumarase Antibody (7612) [NBP2-43578]

Western Blot: Fumarase Antibody (7612) [NBP2-43578] - Analysis of A. 50 ug rat liver lysate/extract 10 % SDS-PAGE Fumarate hydratase antibody [7612] dilution: 1:1000
Fumarase Antibody (7612) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Fumarase Antibody (7612) [NBP2-43578] -

Fumarate hydratase antibody [GT7612] detects Fumarate hydratase protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Fumarate hydratase protein stained by Fumarate hydratase antibody [GT7612] (NBP2-43578) diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

Applications for Fumarase Antibody (7612) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Fumarase

Fumarase is encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy.

Alternate Names

EC 4.2.1.2, Fumarase, fumarate hydratase, fumarate hydratase, mitochondrial, HLRCC, LRCC, MCL, MCUL1

Gene Symbol

FH

Additional Fumarase Products

Product Documents for Fumarase Antibody (7612) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Fumarase Antibody (7612) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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