Fumarase Antibody (OTI1F10)

Novus Biologicals | Catalog # NBP1-47754

Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine

Cited:

Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1F10
View all Fumarase Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human FH (NP_000134) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody is specific for Homo sapiens fumarate hydratase (FH), nuclear gene encoding mitochondrial protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

50.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Fumarase Antibody (OTI1F10)

Western Blot: Fumarase Antibody (1F10) [NBP1-47754] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-Fumarase monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunocytochemistry/ Immunofluorescence: Fumarase Antibody (OTI1F10) [NBP1-47754]

Immunocytochemistry/ Immunofluorescence: Fumarase Antibody (OTI1F10) [NBP1-47754]

Immunocytochemistry/Immunofluorescence: Fumarase Antibody (1F10) [NBP1-47754] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Fumarase.
Immunohistochemistry: Fumarase Antibody (OTI1F10) [NBP1-47754]

Immunohistochemistry: Fumarase Antibody (OTI1F10) [NBP1-47754]

Immunohistochemistry: Fumarase Antibody (OTI1F10) [NBP1-47754] - Fumarase Antibody (1F10) [NBP1-47754] - Staining of paraffin-embedded Human colon tissue using anti-Fumarase mouse monoclonal antibody.
Flow Cytometry: Fumarase Antibody (OTI1F10) [NBP1-47754]

Flow Cytometry: Fumarase Antibody (OTI1F10) [NBP1-47754]

Flow Cytometry: Fumarase Antibody (1F10) [NBP1-47754] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Fumarase antibody, and then analyzed by flow cytometry.
Western Blot: Fumarase Antibody (OTI1F10) [NBP1-47754]

Western Blot: Fumarase Antibody (OTI1F10) [NBP1-47754]

Western Blot: Fumarase Antibody (1F10) [NBP1-47754] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Fumarase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Fumarase.
Immunohistochemistry-Paraffin: Fumarase Antibody (OTI1F10) [NBP1-47754]

Immunohistochemistry-Paraffin: Fumarase Antibody (OTI1F10) [NBP1-47754]

Immunohistochemistry-Paraffin: Fumarase Antibody (1F10) [NBP1-47754] - Staining of paraffin-embedded Human endometrium tissue using anti-Fumarase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Fumarase Antibody (OTI1F10) [NBP1-47754]

Immunohistochemistry-Paraffin: Fumarase Antibody (OTI1F10) [NBP1-47754]

Immunohistochemistry-Paraffin: Fumarase Antibody (1F10) [NBP1-47754] - Staining of paraffin-embedded Human Kidney tissue using anti-Fumarase mouse monoclonal antibody.

Applications for Fumarase Antibody (OTI1F10)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:1000

Flow Cytometry Panel Builder

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Advanced Features

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  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Fumarase

The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form,differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion,where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy.

Alternate Names

EC 4.2.1.2, Fumarase, fumarate hydratase, fumarate hydratase, mitochondrial, HLRCC, LRCC, MCL, MCUL1

Entrez Gene IDs

2271 (Human); 24368 (Rat)

Gene Symbol

FH

UniProt

P07954 (Human)

Additional Fumarase Products

Product Documents for Fumarase Antibody (OTI1F10)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Fumarase Antibody (OTI1F10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Fumarase Antibody (OTI1F10)

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Fumarase Antibody (OTI1F10)

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  • Q: I'm going to be using this antibody for Immunohistochemistry. Can I use a secondary antibody directly conjugated with the HRP, without the biotin/avidin system? Would the sensitivity be drastically affected? The PBS working concentration is 1X, right (PBS 1X + 0.1% Triton X-100, pH 7.4)? Which will be the same used for primary antibody? Which is the most adequate HIER protocol for this antibody? Can you provide a list of references where they show satisfactory results using this antibody?

    A: You may use NBP1-47754 with a secondary that is directly conjugated to HRP, but the sensitivity will be reduced. Using a biotin/strepavidin system allows greater staining essentially because multiple enzymes associate with each primary antibody. If you already have an HRP-conjugated secondary, I would start with that but keep in mind that you may need to change your detection system if you get no signal or weak staining. I'm sorry but we don't have any peer-reviewed references for this product. Here is the recommended protocol for antigen retrieval: Antigen Retrieval Solution: 0.01M Sodium Citrate Buffer, pH 6.0 To prepare stock solutions: Solution A. 0.1 M citric acid solution: dissolve 21.0 g of citric acid, monohydrate (C6H8O7.H2O) in 100 ml of dH2O. Solution B. 0.1M sodium citrate solution: dissolve 29.4 g trisodium citrate dihydrate (C6H5Na3O7.2H2O) in 100 ml of dH2O. Working solution: Add 9 ml of Stock solution A and 41 ml of stock solution B to 450 ml of dH2O. Adjust pH to 6.0.

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