FUS Antibody (HL2454) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25471

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2454 expressed in HEK293

Format

Azide and BSA Free
View all FUS Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human FUS.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

72 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FUS Antibody (HL2454) - Azide and BSA Free

FUS Antibody (HL2454) - Azide and BSA Free

Western Blot: FUS Antibody (HL2454) - Azide and BSA Free [NBP3-25471] -

Western Blot: FUS Antibody (HL2454) - Azide and BSA Free [NBP3-25471] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with FUS antibody [HL2454] (NBP3-25471) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
FUS Antibody (HL2454) - Azide and BSA Free

Immunohistochemistry-Paraffin: FUS Antibody (HL2454) - Azide and BSA Free [NBP3-25471] -

Immunohistochemistry-Paraffin: FUS Antibody (HL2454) - Azide and BSA Free [NBP3-25471] - FUS antibody [HL2454] detects FUS protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. FUS stained by FUS antibody [HL2454] (NBP3-25471) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
FUS Antibody (HL2454) - Azide and BSA Free

Western Blot: FUS Antibody (HL2454) - Azide and BSA Free [NBP3-25471] -

Western Blot: FUS Antibody (HL2454) - Azide and BSA Free [NBP3-25471] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with FUS antibody [HL2454] (NBP3-25471) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
FUS Antibody (HL2454) - Azide and BSA Free

Western Blot: FUS Antibody (HL2454) - Azide and BSA Free [NBP3-25471] -

Western Blot: FUS Antibody (HL2454) - Azide and BSA Free [NBP3-25471] - Wild-type (WT) and FUS knockout (KO) 293T cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FUS antibody [HL2454] (NBP3-25471) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
FUS Antibody (HL2454) - Azide and BSA Free

Immunohistochemistry-Paraffin: FUS Antibody (HL2454) - Azide and BSA Free [NBP3-25471] -

Immunohistochemistry-Paraffin: FUS Antibody (HL2454) - Azide and BSA Free [NBP3-25471] - FUS antibody [HL2454] detects FUS protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. FUS stained by FUS antibody [HL2454] (NBP3-25471) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
FUS Antibody (HL2454) - Azide and BSA Free

ICC/IF-FUS Antibody (HL2454) - Azide and BSA Free-NBP3-25471-

ICC/IF-FUS Antibody (HL2454) - Azide and BSA Free-NBP3-25471-HepG2 cells were fixed in 4% PFA.Green: FUS stained by FUS antibody [HL2454]diluted at 1:500.Blue: Fluoroshield with DAPI.

Applications for FUS Antibody (HL2454) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Assay dependent

Immunohistochemistry

Assay dependent

Immunohistochemistry-Paraffin

Assay dependent

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FUS

FUS, fused in sarcoma, encodes a multifunctional protein component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex. Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Coactivates expression in an agonist- and AF2-dependent manner. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ERs), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Probably functions as a general coactivator, rather than just a nuclear receptor coactivator. May also be involved in the coactivation of the NF-kappa-B pathway. May coactivate expression via a remodeling of chromatin and its interaction with histone acetyltransferase proteins. Involved in placental, cardiac, hepatic and embryonic development. NCOA6 is over-expressed in several breast cancer cell lines.

Long Name

FUS RNA binding protein

Alternate Names

ALS6, altFUS, ETM4, FUS1, HNRNPP2, POMP75, TLS

Gene Symbol

FUS

Additional FUS Products

Product Documents for FUS Antibody (HL2454) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for FUS Antibody (HL2454) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for FUS Antibody (HL2454) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review FUS Antibody (HL2454) - Azide and BSA Free and earn rewards!

Have you used FUS Antibody (HL2454) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies