Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Cited:

Immunoprecipitation, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all FUS Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide which maps to a region between residues 400 and 450 of human fusion (involved in t(12;16) in malignant liposarcoma) using the numbering given in SwissProt entry P35637 (GeneID 2521).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit FUS Antibody - BSA Free (NB100-561) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-FUS Antibody: Cited in 11 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for FUS Antibody - BSA Free

Western Blot: FUS Antibody [NB100-561]

Western Blot: FUS Antibody [NB100-561]

Western Blot: FUS Antibody [NB100-561] - Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbitanti-FUS antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with anexposure time of 3 seconds.
Immunohistochemistry-Paraffin: FUS Antibody [NB100-561]

Immunohistochemistry-Paraffin: FUS Antibody [NB100-561]

Immunohistochemistry-Paraffin: FUS Antibody [NB100-561] - Detection of mouse FUS by immunohistochemistry. Sample: FFPE section of mouse teratoma Antibody: Affinity purified rabbit anti-FUS (NB100-561) used at 1:5,000 (0.2ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: FUS Antibody [NB100-561]

Immunohistochemistry-Paraffin: FUS Antibody [NB100-561]

Immunohistochemistry-Paraffin: FUS Antibody [NB100-561] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-FUS (NB100-561). Detection: DAB
Immunoprecipitation: FUS Antibody [NB100-561]

Immunoprecipitation: FUS Antibody [NB100-561]

Immunoprecipitation: FUS Antibody [NB100-561] - FUS immunoprecipitation in human fibroblast cell lysates. Image from verified customer review.
Immunoprecipitation: FUS Antibody [NB100-561]

Immunoprecipitation: FUS Antibody [NB100-561]

Immunoprecipitation: FUS Antibody [NB100-561] - Detection of human FUS by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FUS antibody NB100-561 used for IP at 3 ug per reaction. FUS was also immunoprecipitated by rabbit anti-FUS recombinant monoclonal antibody [BLR023E] (NBP2-76415) and rabbit anti-FUS antibody NB100-565. For blotting immunoprecipitated FUS, NBP2-76415 was used at 1:1000. Detection: Chemiluminescence with an exposure time time of 3 minutes.

Applications for FUS Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:1000-1:5000

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000 - 1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Reviewed Applications

Read 3 reviews rated 5 using NB100-561 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: FUS

FUS is a nucleic acid-binding protein whose N-terminal half functions as a transcriptional activator domain in fusion oncoproteins found in human leukemias and liposarcomas. FUS has been reported to be associated with the nuclear matrix and has been implicated in transcription, pre-mRNA splicing processes, spliceosome assembly, and promoting undifferentiated endothelial cells.

Alternate Names

75 kDa DNA-pairing protein, ALS6, amyotrophic lateral sclerosis 6, FUS1, fused in sarcoma, fusion (involved in t(12;16) in malignant liposarcoma), fusion gene in myxoid liposarcoma, fusion, derived from t(12;16) malignant liposarcoma, fus-like protein, heterogeneous nuclear ribonucleoprotein P2, HNRNPP2, Oncogene FUS, Oncogene TLS, POMp75, RNA-binding protein FUS, TLShnRNP-P2, Translocated in liposarcoma protein

Entrez Gene IDs

2521 (Human)

Gene Symbol

FUS

UniProt

P35637 (Human)

Additional FUS Products

Product Documents for FUS Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for FUS Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for FUS Antibody - BSA Free

Customer Reviews for FUS Antibody - BSA Free (3)

5 out of 5
3 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • FUS Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: fibroblasts
    Species: Human
    Verified Customer | Posted 11/18/2017
    FUS in fibroblasts Blocked 1H 25C in 1X PBS/5% normal serum/0.3% Triton™ X-100. Antibody diluted 1:200 in 1X PBS/1% BSA/0.3% Triton™ X-100, 16 hours at 4C incubation.
    Blocked 1H 25C in 1X PBS/5% normal serum/0.3% Triton™ X-100. Antibody diluted 1:200 in 1X PBS/1% BSA/0.3% Triton™ X-100, 16 hours at 4C incubation.
    FUS Antibody - BSA Free NB100-561
  • Name: Anonymous
    Application: Immunoprecipitation
    Sample Tested: Human fibroblasts, whole cell lysate
    Species: Human
    Verified Customer | Posted 11/26/2014
    FUS immunoprecipitation in human fibroblasts
    FUS Antibody - BSA Free NB100-561
  • Name: Jennifer Fox
    Application: Western Blot
    Sample Tested: Human fibroblasts, whole cell lysate
    Species: Human
    Verified Customer | Posted 10/17/2014
    FUS expression in human fibroblasts
    FUS Antibody - BSA Free NB100-561

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Protocols

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