FUS Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89113

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all FUS Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SSQSSYGQQSSYPGYGQQPAPSSTSGSYGSSSQSSSYGQPQSGSYSQQPSYGGQQQSYGQQQSYNPPQGYGQQNQYNSSSGGGGGGGGGGNYGQDQSSMSSGGGSGGGYGNQDQSGGGGSGGYGQQDR

Reactivity Notes

Reactivity reported in scientific literature (PMID: 19669651)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit FUS Antibody - BSA Free (NBP1-89113) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-FUS Antibody: Cited in 19 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for FUS Antibody - BSA Free

Immunohistochemistry-Paraffin: FUS Antibody [NBP1-89113]

Immunohistochemistry-Paraffin: FUS Antibody [NBP1-89113]

Immunohistochemistry-Paraffin: FUS Antibody [NBP1-89113] - Staining in human endometrium and liver tissues. Corresponding FUS RNA-seq data are presented for the same tissues.
Western Blot: FUS Antibody [NBP1-89113]

Western Blot: FUS Antibody [NBP1-89113]

Western Blot: FUS Antibody [NBP1-89113] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-FUS antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: FUS Antibody [NBP1-89113]

Immunocytochemistry/ Immunofluorescence: FUS Antibody [NBP1-89113]

Immunocytochemistry/Immunofluorescence: FUS Antibody [NBP1-89113] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
Western Blot: FUS Antibody [NBP1-89113]

Western Blot: FUS Antibody [NBP1-89113]

Western Blot: FUS Antibody [NBP1-89113] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: FUS Antibody [NBP1-89113]

Immunohistochemistry-Paraffin: FUS Antibody [NBP1-89113]

Immunohistochemistry-Paraffin: FUS Antibody [NBP1-89113] - Staining of human cerebellum shows strong nuclear positivity in neuronal cells.
Immunohistochemistry-Paraffin: FUS Antibody [NBP1-89113]

Immunohistochemistry-Paraffin: FUS Antibody [NBP1-89113]

Immunohistochemistry-Paraffin: FUS Antibody [NBP1-89113] - Staining of human colon shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: FUS Antibody [NBP1-89113]

Immunohistochemistry-Paraffin: FUS Antibody [NBP1-89113]

Immunohistochemistry-Paraffin: FUS Antibody [NBP1-89113] - Staining of human endometrium shows strong nuclear positivity in glandular cells and stromal cells.
Immunohistochemistry-Paraffin: FUS Antibody [NBP1-89113]

Immunohistochemistry-Paraffin: FUS Antibody [NBP1-89113]

Immunohistochemistry-Paraffin: FUS Antibody [NBP1-89113] - Staining of human liver shows moderate nuclear positivity in bile duct cells while hepatocytes are negative.
Immunohistochemistry-Paraffin: FUS Antibody [NBP1-89113]

Immunohistochemistry-Paraffin: FUS Antibody [NBP1-89113]

Immunohistochemistry-Paraffin: FUS Antibody [NBP1-89113] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.

Applications for FUS Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000-1:2500

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization, PFA/Triton X-100. FUS antibody validated for IHC-P from a verified customer review.

Reviewed Applications

Read 1 review rated 5 using NBP1-89113 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FUS

FUS, fused in sarcoma, encodes a multifunctional protein component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex. Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Coactivates expression in an agonist- and AF2-dependent manner. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ERs), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Probably functions as a general coactivator, rather than just a nuclear receptor coactivator. May also be involved in the coactivation of the NF-kappa-B pathway. May coactivate expression via a remodeling of chromatin and its interaction with histone acetyltransferase proteins. Involved in placental, cardiac, hepatic and embryonic development. NCOA6 is over-expressed in several breast cancer cell lines.

Alternate Names

75 kDa DNA-pairing protein, ALS6, amyotrophic lateral sclerosis 6, FUS1, fused in sarcoma, fusion (involved in t(12;16) in malignant liposarcoma), fusion gene in myxoid liposarcoma, fusion, derived from t(12;16) malignant liposarcoma, fus-like protein, heterogeneous nuclear ribonucleoprotein P2, HNRNPP2, Oncogene FUS, Oncogene TLS, POMp75, RNA-binding protein FUS, TLShnRNP-P2, Translocated in liposarcoma protein

Gene Symbol

FUS

Additional FUS Products

Product Documents for FUS Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for FUS Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for FUS Antibody - BSA Free

Customer Reviews for FUS Antibody - BSA Free (1)

5 out of 5
1 Customer Rating
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4 Stars
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  • FUS Antibody
    Name: Kristi Bailey
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Human brain
    Species: Human
    Verified Customer | Posted 01/26/2018
    FFPE 5micron sections, Citrate pretreatment, 1:500 20m, Ultravision DAB detection
    FUS Antibody - BSA Free NBP1-89113

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