FUS Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-03651

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Knockout Validated, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all FUS Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 297-526 of human FUS (NP_004951.1). TIESVADYFKQIGIIKTNKKTGQPMINLYTDRETGKLKGEATVSFDDPPSAKAAIDWFDGKEFSGNPIKVSFATRRADFNRGGGNGRGGRGRGGPMGRGGYGGGGSGGGGRGGFPSGGGGGGGQQRAGDWKCPNPTCENMNFSWRNECNQCKAPKPDGPGGGPGGSHMGGNYGDDRRGGRGGYDRGGYRGRGGDRGGFRGGRGGGDRGGFGPGKMDSRGEHRQDRRERPY

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FUS Antibody - BSA Free

Knockout Validated: FUS Antibody - BSA Free [NBP3-03651]

Western Blot: FUS Antibody - BSA Free [NBP3-03651]

Western Blot: FUS Antibody [NBP3-03651] - Analysis of extracts from normal (control) and FUS knockout (KO) 293T cells, using FUS antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunoprecipitation: FUS Antibody - BSA Free [NBP3-03651]

Immunoprecipitation: FUS Antibody - BSA Free [NBP3-03651]

Immunoprecipitation: FUS Antibody [NBP3-03651] - Analysis of 300ug extracts of Jurkat cells using 3ug FUS antibody. Western blot was performed from the immunoprecipitate using FUS antibody at a dilition of 1:2000.
FUS Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: FUS Antibody - BSA Free [NBP3-03651] -

Immunocytochemistry/ Immunofluorescence: FUS Antibody - BSA Free [NBP3-03651] - Immunofluorescence analysis of C6 cells using [KO Validated] FUS Rabbit pAb (A5921) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
FUS Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: FUS Antibody - BSA Free [NBP3-03651] -

Immunocytochemistry/ Immunofluorescence: FUS Antibody - BSA Free [NBP3-03651] - Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] FUS Rabbit pAb (A5921) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for FUS Antibody - BSA Free

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL.

Immunocytochemistry/ Immunofluorescence

1:50 - 1:100

Immunoprecipitation

0.5μg-4μg antibody for 200μg-400μg extracts of whole cells.

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FUS

FUS, fused in sarcoma, encodes a multifunctional protein component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex. Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Coactivates expression in an agonist- and AF2-dependent manner. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ERs), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Probably functions as a general coactivator, rather than just a nuclear receptor coactivator. May also be involved in the coactivation of the NF-kappa-B pathway. May coactivate expression via a remodeling of chromatin and its interaction with histone acetyltransferase proteins. Involved in placental, cardiac, hepatic and embryonic development. NCOA6 is over-expressed in several breast cancer cell lines.

Long Name

FUS RNA binding protein

Alternate Names

ALS6, altFUS, ETM4, FUS1, HNRNPP2, POMP75, TLS

Gene Symbol

FUS

Additional FUS Products

Product Documents for FUS Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for FUS Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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