FXR1 Antibody

Novus Biologicals | Catalog # NB100-41388

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all FXR1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-RIEGDNENKLPRED corresponding to internal region according to NP_001013456.1, NP_001013457.1.

Reactivity Notes

Expected from sequence similarity: Human, Rat, Canine

Specificity

This antibody is expected to recognise all reported isoforms (NP_005078.2, NP_001013456.1 and NP_001013457.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for FXR1 Antibody

Western Blot: FXR1 Antibody [NB100-41388]

Western Blot: FXR1 Antibody [NB100-41388]

Western Blot: FXR1 Antibody [NB100-41388] - 1ug/ml staining of NIH-3T3 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: FXR1 Antibody [NB100-41388]

Immunocytochemistry/ Immunofluorescence: FXR1 Antibody [NB100-41388]

Immunocytochemistry/Immunofluorescence: FXR1 Antibody [NB100-41388] - Paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary antibody at 10 ug/mL, 1 hr incubation followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunocytochemistry/ Immunofluorescence: FXR1 Antibody [NB100-41388]

Immunocytochemistry/ Immunofluorescence: FXR1 Antibody [NB100-41388]

Immunocytochemistry/Immunofluorescence: FXR1 Antibody [NB100-41388] - Paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary antibody at 10 ug/mL, 1 hr incubation, followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Applications for FXR1 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:128000

Western Blot

1:1000
Application Notes
Western blot: Approx 75+80kDa band observed in lysates of cell line NIH-3T3 (calculated MW of 59.9 kDa according to NP_001013457.1). The observed molecular weight corresponds to earlier findings in literature (Khandjian et al Hum Mol Genet. 1998 Dec;7(13):2121-8.; PMID: 9817930). An additional band of unknown identity was also consistently observed at 37 kDa. This band was successfully blocked by incubation with the immunizing peptide.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FXR1

The protein encoded by this gene is an RNA binding protein that interacts with the functionally-similar proteins FMR1 and FXR2. These proteins shuttle between the nucleus and cytoplasm and associate with polyribosomes, predominantly with the 60S ribosomal subunit. Three transcript variants encoding different isoforms have been found for this gene.

Alternate Names

fragile X mental retardation syndrome-related protein 1, fragile X mental retardation, autosomal homolog 1, FXR1P, hFXR1p

Entrez Gene IDs

8087 (Human); 14359 (Mouse); 361927 (Rat)

Gene Symbol

FXR1

UniProt

NP_005078.2 (Human)

Additional FXR1 Products

Product Documents for FXR1 Antibody

Certificate of Analysis

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Product Specific Notices for FXR1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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