Fyn Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-82685

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Fyn Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SFGVTSIPNYNNFHAAGGQGLTVFGGVNSSSHTGTLRTRGGTGVTLFVALYDYEARTEDDLSFHKGEKFQILNSSEGDWWEARSLTTGETGYIPSNYVAPVDSIQAEEWYFGKLGRKDAERQLLSFGNPRGTFLIRESETTKGAYSLSIR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit Fyn Antibody - BSA Free (NBP1-82685) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Fyn Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Fyn Antibody - BSA Free

Western Blot: Fyn Antibody [NBP1-82685]

Western Blot: Fyn Antibody [NBP1-82685]

Western Blot: Fyn Antibody [NBP1-82685] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: Fyn Antibody [NBP1-82685]

Immunocytochemistry/ Immunofluorescence: Fyn Antibody [NBP1-82685]

Immunocytochemistry/Immunofluorescence: Fyn Antibody [NBP1-82685] - Staining of human cell line AF22 shows localization to plasma membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Fyn Antibody [NBP1-82685]

Immunohistochemistry-Paraffin: Fyn Antibody [NBP1-82685]

Immunohistochemistry-Paraffin: Fyn Antibody [NBP1-82685] - Staining of human tonsil shows strong cytoplasmic positivity in a subset of reaction center cells and lymphoid cells outside reaction centra.
Western Blot: Fyn Antibody [NBP1-82685]

Western Blot: Fyn Antibody [NBP1-82685]

Western Blot: Fyn Antibody [NBP1-82685] - Analysis in human cell line U-87 MG.
Western Blot: Fyn Antibody [NBP1-82685]

Western Blot: Fyn Antibody [NBP1-82685]

Western Blot: Fyn Antibody [NBP1-82685] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Fyn Antibody

Immunohistochemistry-Paraffin: Fyn Antibody [NBP1-82685] -

Immunohistochemistry-Paraffin: Fyn Antibody [NBP1-82685] -Staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil.
Fyn Antibody

Immunohistochemistry-Paraffin: Fyn Antibody [NBP1-82685] -

Immunohistochemistry-Paraffin: Fyn Antibody [NBP1-82685] - Staining of human kidney shows moderate cytoplasmic positivity in cells in glomeruli.
Fyn Antibody

Immunohistochemistry-Paraffin: Fyn Antibody [NBP1-82685] -

Immunohistochemistry-Paraffin: Fyn Antibody [NBP1-82685] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Applications for Fyn Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Fyn

Fyn is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist.

Long Name

FYN oncogene related to SRC, FGR, YES

Alternate Names

SLK, SYN

Gene Symbol

FYN

Additional Fyn Products

Product Documents for Fyn Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Fyn Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Fyn Antibody - BSA Free

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Protocols

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FAQs

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Associated Pathways

VEGF - VEGF R2 Signaling Pathways VEGF - VEGF R2 Signaling Pathway Thumbnail