FZR1/CDH1 Antibody

Novus Biologicals | Catalog # NBP2-15840

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Predicted:

Bovine (97%), Chicken (94%), Porcine (97%), Rhesus Macaque (100%), Xenopus (90%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all FZR1/CDH1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human FZR1/CDH1. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (90%).

Localization

Isoform 2: Nucleus; Isoform 3: Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FZR1/CDH1 Antibody

Western Blot: FZR1/CDH1 Antibody [NBP2-15840]

Western Blot: FZR1/CDH1 Antibody [NBP2-15840]

FZR1-CDH1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15840-img0014.jpg
Immunohistochemistry-Paraffin: FZR1/CDH1 Antibody [NBP2-15840]

Immunohistochemistry-Paraffin: FZR1/CDH1 Antibody [NBP2-15840]

Immunohistochemistry-Paraffin: FZR1/CDH1 Antibody [NBP2-15840] - Mouse brain. FZR1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: FZR1/CDH1 Antibody [NBP2-15840]

Western Blot: FZR1/CDH1 Antibody [NBP2-15840]

Western Blot: FZR1/CDH1 Antibody [NBP2-15840] - A431 whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with FZR1/CDH1 antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: FZR1/CDH1 Antibody [NBP2-15840]

Immunohistochemistry-Paraffin: FZR1/CDH1 Antibody [NBP2-15840]

Immunohistochemistry-Paraffin: FZR1/CDH1 Antibody [NBP2-15840] - Mouse heart. FZR1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: FZR1/CDH1 Antibody [NBP2-15840]

Immunohistochemistry-Paraffin: FZR1/CDH1 Antibody [NBP2-15840]

Immunohistochemistry-Paraffin: FZR1/CDH1 Antibody [NBP2-15840] - Rat heart. FZR1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
FZR1/CDH1 Antibody

Immunocytochemistry/ Immunofluorescence: FZR1/CDH1 Antibody [NBP2-15840] -

FZR1 antibody detects FZR1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FZR1 protein stained by FZR1 antibody (NBP2-15840) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar = 10 um.

Applications for FZR1/CDH1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100 - 1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: FZR1/CDH1

Key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis.

Alternate Names

CDC20C, CDH1, Cdh1/Hct1 homolog, CDH1CDC20-like 1b, fizzy/cell division cycle 20 related 1 (Drosophila), Fzr, FZR2, FZRCDC20-like protein 1, hCDH1, HCDHFYR, KIAA1242fizzy-related protein homolog

Gene Symbol

FZR1

UniProt

Q9UM11 (Human)

Additional FZR1/CDH1 Products

Product Documents for FZR1/CDH1 Antibody

Certificate of Analysis

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Product Specific Notices for FZR1/CDH1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for FZR1/CDH1 Antibody

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Protocols

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