G-CSFR/CD114 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-16629

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all G-CSFR/CD114 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human G-CSFR/CD114. The exact sequence is proprietary.

Localization

Isoform 2: Secreted; Cell membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

92 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit G-CSFR/CD114 Antibody - BSA Free (NBP2-16629) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for G-CSFR/CD114 Antibody - BSA Free

Western Blot: G-CSFR/CD114 Antibody [NBP2-16629]

Western Blot: G-CSFR/CD114 Antibody [NBP2-16629]

Western Blot: G-CSFR/CD114 Antibody [NBP2-16629] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with G-CSFR antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: G-CSFR/CD114 Antibody [NBP2-16629]

Immunohistochemistry-Paraffin: G-CSFR/CD114 Antibody [NBP2-16629]

Immunohistochemistry-Paraffin: G-CSF R/CD114 Antibody [NBP2-16629] - Paraffin-embedded human breast carcinoma. GCSF Receptor antibody [N2C1], Internal diluted at 1:250.
G-CSFR/CD114 Antibody [NBP2-16629] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GCSF Receptor antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
G-CSFR/CD114 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: G-CSFR/CD114 Antibody [NBP2-16629] -

GCSF Receptor antibody [N2C1], Internal detects GCSF Receptor protein at cell membrane by immunofluorescent analysis.
Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GCSF Receptor stained by GCSF Receptor antibody [N2C1], Internal (NBP2-16629) diluted at 1:500.
Blue: Fluoroshield with DAPI.

Applications for G-CSFR/CD114 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: G-CSFR/CD114

The protein encoded by this gene is the receptor for colony stimulating factor 3, a cytokine that controls the production, differentiation, and function of granulocytes. The encoded protein, which is a member of the family of cytokine receptors, may also function in some cell surface adhesion or recognition processes. Four transcript variants encoding four different isoforms have been found for this gene, with three of the isoforms being membrane-bound and the other being secreted and soluble. Mutations in this gene are a cause of Kostmann syndrome, also known as severe congenital neutropenia. [provided by RefSeq]

Long Name

Granulocyte Colony Stimulating Factor Receptor

Alternate Names

CD114, CSF3R, Csfgr, G-CSF R, GCSFR

Gene Symbol

CSF3R

UniProt

Q99062 (Human)

Additional G-CSFR/CD114 Products

Product Documents for G-CSFR/CD114 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for G-CSFR/CD114 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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