G protein alpha-13 Antibody - BSA Free
Novus Biologicals | Catalog # NBP3-12983
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Key Product Details
Validated by
Knockout/Knockdown, Independent Antibodies
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human G protein alpha-13. The exact sequence is proprietary.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Centrifuge briefly prior to opening.
Scientific Data Images for G protein alpha-13 Antibody - BSA Free
Western Blot: G protein alpha-13 Antibody [NBP3-12983]
Western Blot: G protein alpha-13 Antibody [NBP3-12983] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with G protein alpha-13 antibody (NBP3-12983) diluted at 1:1000 and competitor's antibody diluted by 1:50.Immunohistochemistry-Paraffin: G protein alpha-13 Antibody [NBP3-12983]
Immunohistochemistry-Paraffin: G protein alpha-13 Antibody [NBP3-12983] - G protein alpha-13 antibody detects G protein alpha-13 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. G protein alpha-13 stained by G protein alpha-13 antibody (NBP3-12983) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 minWestern Blot: G protein alpha-13 Antibody [NBP3-12983]
Western Blot: G protein alpha-13 Antibody [NBP3-12983] - Wild-type (WT) and G protein alpha-13 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with G protein alpha-13 antibody (NBP3-12983) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Immunohistochemistry-Paraffin: G protein alpha-13 Antibody [NBP3-12983]
Immunohistochemistry-Paraffin: G protein alpha-13 Antibody [NBP3-12983] - G protein alpha-13 antibody detects G protein alpha-13 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. G protein alpha-13 stained by G protein alpha-13 antibody (NBP3-12983) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 minApplications for G protein alpha-13 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Format
BSA Free
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: G protein alpha-13
Alternate Names
G alpha-13, G13, G-protein subunit alpha-13, guanine nucleotide binding protein (G protein), alpha 13, guanine nucleotide-binding protein subunit alpha-13, MGC46138
Gene Symbol
GNA13
Additional G protein alpha-13 Products
Product Documents for G protein alpha-13 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for G protein alpha-13 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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