G protein beta 4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-13295

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Mouse (100%), Rat (100%), Rhesus Macaque (100%), Xenopus (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all G protein beta 4 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse G protein beta 4. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for G protein beta 4 Antibody - BSA Free

Western Blot: G protein beta 4 Antibody [NBP3-13295]

Western Blot: G protein beta 4 Antibody [NBP3-13295]

Western Blot: G protein beta 4 Antibody [NBP3-13295] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with G protein beta 4 antibody (NBP3-13295) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: G protein beta 4 Antibody [NBP3-13295]

Immunohistochemistry-Paraffin: G protein beta 4 Antibody [NBP3-13295]

Immunohistochemistry-Paraffin: G protein beta 4 Antibody [NBP3-13295] - G protein beta 4 antibody detects G protein beta 4 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian cancer. G protein beta 4 stained by G protein beta 4 antibody (NBP3-13295) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: G protein beta 4 Antibody [NBP3-13295]

Immunohistochemistry-Paraffin: G protein beta 4 Antibody [NBP3-13295]

Immunohistochemistry-Paraffin: G protein beta 4 Antibody [NBP3-13295] - G protein beta 4 antibody detects G protein beta 4 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. G protein beta 4 stained by G protein beta 4 antibody (NBP3-13295) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
G protein beta 4 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: G protein beta 4 Antibody [NBP3-13295] -

G protein beta antibody detects G protein beta protein at cell membrane and cytoplasm by immunofluorescent analysis.
Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: G protein beta protein stained by G protein beta antibody (NBP3-13295) diluted at 1:500.
Blue: Hoechst 33342 staining.

Applications for G protein beta 4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: G protein beta 4

FUNCTION: Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. SUBUNIT: G proteins are composed of 3 units, alpha, beta and gamma. TISSUE SPECIFICITY: Strongly expressed in lung and placenta, whereas it is weakly expressed in brain and heart.

Alternate Names

G protein beta-4 subunit, guanine nucleotide binding protein (G protein), beta polypeptide 4, guanine nucleotide binding protein beta subunit 4, guanine nucleotide-binding protein subunit beta-4, Transducin beta chain 4

Gene Symbol

GNB4

Additional G protein beta 4 Products

Product Documents for G protein beta 4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for G protein beta 4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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