GABA-A-R beta 1 C-Terminus Antibody

Name: GABA-A-R beta 1 C-Terminus Antibody
Brand: R&D Systems
SKU: PPS030

Catalog #: PPS030 Datasheet / COA / SDS

Newer Version Available: PPS030B

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Discontinued Product

PPS030 has been discontinued and is replaced by PPS030B.

Detection of GABAAR beta 1 C-Terminus by Western Blot.

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All GABA-A R beta 1 Antibodies

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GABA-A-R beta 1 C-Terminus Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Human, mouse, and rat ~55 kDa GABA_A R beta 1 subunit, C-Terminus

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

Fusion protein from the cytosolic loop of the rat GABA_A R beta 1 subunit, C-Terminus

Formulation

100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1:1000 dilution

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of GABA_AR beta 1 C-Terminus by Western Blot. Western blot of 7 μg of rat cerebellar and hippocampal lysates showing specific immunolabeling of the ~55 kDa beta 1 subunit of the GABA_AR.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: GABA-A R beta 1

GABA_A ( gamma -aminobutyric acid-type A) receptors are members of the cysteine-loop family of neurotransmitter-gated ion channels. GABA binding to A-type receptors induces anion-selective ion channel opening. These receptors are the principal fast inhibitory neurotransmitter receptors in the CNS. GABA_A receptors are heteropentamer combinations of seven subunit types; alpha, beta, gamma, δ, epsilon, theta, and π. Three subunits, alpha, beta, and gamma, have at least three separate gene products in mammals, and typical GABA_A receptors have some combination of alpha, beta and gamma subunits. The rat beta 1 isoform is a 58 - 60 kDa, 449 amino acid (aa), 4 transmembrane protein with two terminal extracellular regions. The ligand-binding region is in the N-terminus (aa 13 - 218). The beta 1 subunit is known to be phosphorylated on a consensus phosphorylation site (S409 of the precursor) that exists in the cytoplasmic domain between transmembrane segments 3 and 4. PKA or PKC beta II-induced phosphorylation decreases GABA_A receptor activity.

References

Darlison, M.G. et al. (2005) Cell. Mol. Neurobiol. 25:607.
Akabas, M.H. (2004) Int. Rev. Neurobiol. 62:1.
Song, M. and R.O. Messing (2005) Cell. Mol. Life Sci. 62:119.
Hinkle, D.J. and R.L. MacDonald (2003) J. Neurosci. 23:11698.
Brandon, N.J. et al. (2003) Mol. Cell. Neurosci. 22:87.

Long Name

gamma-Aminobutyric Acid Type A Receptor, beta-1 Polypeptide

Entrez Gene IDs

2560 (Human); 14400 (Mouse); 25450 (Rat); 282239 (Bovine)

Alternate Names

GABA A R beta 1; GABA(A) receptor subunit beta-1; GABAAR beta 1; GABAARb1; GABRB1; gamma-aminobutyric acid (GABA) A receptor, beta 1; gamma-aminobutyric acid receptor subunit beta-1