GABA-A R delta Antibody - N-Terminus - Azide Free

Novus Biologicals | Catalog # NB300-200

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Knockout Validated, Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free
View all GABA-A R delta Primary Antibodies »

Product Specifications

Immunogen

Fusion protein from the N-terminus of the delta subunit. Accession # P18506

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 22609410).

Localization

Multi-pass membrane protein

Specificity

Specific for endogenous levels of the ~50 kDa delta-subunit of the GABAA receptor.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

As of 1/20/2017, this is a new lot of this antbody. It was produced by the same methods, using the same fusion protein antigen in new animals.

Scientific Data Images for GABA-A R delta Antibody - N-Terminus - Azide Free

Western Blot: GABA-A R delta Antibody [NB300-200]

Western Blot: GABA-A R delta Antibody [NB300-200]

Western Blot: GABA-A R delta Antibody [NB300-200] - mouse whole brain (1) and mouse synaptic plasma membrane (2) lysates showing specific immunolabeling of the Approx. 50 kDa GAmma-subunit of the GABAA-R.

Applications for GABA-A R delta Antibody - N-Terminus - Azide Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:250

Western Blot

1:1000
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 22609410). Knockout Validated reported in scientific literature (PMID: 31209152).

Reviewed Applications

Read 2 reviews rated 4.5 using NB300-200 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Format

Azide Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GABA-A R delta

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA Receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six as, four bs and four gs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for a- and b-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a g-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different a-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Poltl et al., 2003). More recently there have been a number of studies demonstrating that the a-subunit of the receptor may affect subunit assembly (Korpi et al., 2002) and may also confer differential sensitivity to neurosteroids and to ethanol (Wallner et al., 2003; Wohlfarth et al., 2002).

Long Name

gamma-Aminobutyric Acid Type A Receptor, delta Polypeptide

Alternate Names

GABAAR delta, GABAARd, GABRD

Entrez Gene IDs

2563 (Human); 14403 (Mouse); 29689 (Rat)

Gene Symbol

GABRD

UniProt

P18506 (Rat)

Additional GABA-A R delta Products

Product Documents for GABA-A R delta Antibody - N-Terminus - Azide Free

Certificate of Analysis

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Product Specific Notices for GABA-A R delta Antibody - N-Terminus - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

GABA Receptors

Citations for GABA-A R delta Antibody - N-Terminus - Azide Free

Customer Reviews for GABA-A R delta Antibody - N-Terminus - Azide Free (2)

4.5 out of 5
2 Customer Ratings
5 Stars
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4 Stars
50%
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0%
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1 Stars
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  • GABA-A R delta Antibody
    Name: Daniel Popoola
    Application: Western Blot
    Sample Tested: Adolescent and adult cerebellum
    Species: Rat
    Verified Customer | Posted 01/31/2017
    Whole-cell homogenates of cerebellar tissue from adolescent (wells 1,2,5,6,9,10&13 from right to left) and adult ( wells 3,4,7,8,11 &12 from right to left) male Long Evans rats. Ladder labelled as 250, 98, 64, 50, 36 and 30kDa from top to bottom.
    GABA-A R delta Antibody - N-Terminus - Azide Free NB300-200
  • Name: Daniel Popoola
    Application: Western Blot
    Sample Tested: Rat brain synaptosomal (P2) fraction
    Species: Rat
    Verified Customer | Posted 09/26/2014
    GABAA delta subunit quantification in the cerebral cortex rats using western blot
    GABA-A R delta Antibody - N-Terminus - Azide Free NB300-200

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