GABA-AR alpha 2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-02985

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GABA-A R alpha 2 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 300-400 of human GABA-AR alpha 2 (NP_000798.2). ARNSLPKVAYATAMDWFIAVCYAFVFSALIEFATVNYFTKRGWAWDGKSVVNDKKKEKASVMIQNNAYAVAVANYAPNLSKDPVLSTISKSATTPEPNKKP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GABA-AR alpha 2 Antibody - BSA Free

Western Blot: GABA-AR alpha 2 AntibodyBSA Free [NBP3-02985]

Western Blot: GABA-AR alpha 2 AntibodyBSA Free [NBP3-02985]

Western Blot: GABA-AR alpha 2 Antibody [NBP3-02985] - Analysis of extracts of various cell lines, using GABA-AR alpha 2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:Basic ECL Kit
Immunohistochemistry-Paraffin: GABA-AR alpha 2 Antibody - BSA Free [NBP3-02985]

Immunohistochemistry-Paraffin: GABA-AR alpha 2 Antibody - BSA Free [NBP3-02985]

Immunohistochemistry-Paraffin: GABA-AR alpha 2 Antibody [NBP3-02985] - Immunohistochemistry of paraffin-embedded mouse stomach using GABA-AR alpha 2 Rabbit pAb (NBP3-02985) at dilution of 1:250 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Applications for GABA-AR alpha 2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GABA-A R alpha 2

GABA (A) receptors are ligand-gated chloride channels that play a role as inhibitory neurotransmitters. They are known targets for certain classes of environmental and pharmaceutical compounds because a number of drugs interact with binding sites on GABA (A). Some of these drugs include benzodiazepines, anticonvulsants, and anesthetics. GABA (A) Receptors are comprised of subunits and these are further classified into 3 major groups (alpha, beta, and gamma). The subunits determine the pharmacological characteristics.

Long Name

gamma-Aminobutyric Acid Type A Receptor, alpha-2 Polypeptide

Alternate Names

GABAAR alpha 2, GABAARa2, GABRA2

Gene Symbol

GABRA2

Additional GABA-A R alpha 2 Products

Product Documents for GABA-AR alpha 2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for GABA-AR alpha 2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

GABA Receptors

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Protocols

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FAQs

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