GABA-AR alpha 4 Antibody (S398A-34) - BSA Free

Novus Biologicals | Catalog # NBP2-59326

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # S398A-34

Format

BSA Free
View all GABA-A R alpha 4 Primary Antibodies »

Product Specifications

Immunogen

Fusion protein amino acids 410-450 (Cytoplasmic C-terminus) of rat GABA-A-Receptor Alpha 4

Localization

Cell Junction, Synapse, Postsynaptic Cell Membrane, Cell Membrane

Specificity

Detects 60kDa. Does not cross-react with other GABA-A-Receptor Alpha subunits.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for GABA-AR alpha 4 Antibody (S398A-34) - BSA Free

Western Blot: GABA-AR alpha 4 Antibody (S398A-34) [NBP2-59326]

Western Blot: GABA-AR alpha 4 Antibody (S398A-34) [NBP2-59326]

Western Blot: GABA-AR alpha 4 Antibody (S398A-34) [NBP2-59326] - Western Blot analysis of Mouse Brain showing detection of ~60 kDa GABA-A-Receptor-Alpha4 protein using Mouse Anti-GABA-A-Receptor-Alpha4 Monoclonal Antibody, Clone S398A-34 (NBP2-59326). Lane 1: MW Ladder. Lane 2: Mouse Brain. Load: 20 ug. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-GABA-A-Receptor-Alpha4 Monoclonal Antibody (NBP2-59326) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~60 kDa.
Immunocytochemistry/ Immunofluorescence: GABA-AR alpha 4 Antibody (S398A-34) [NBP2-59326]

Immunocytochemistry/ Immunofluorescence: GABA-AR alpha 4 Antibody (S398A-34) [NBP2-59326]

Immunocytochemistry/Immunofluorescence: GABA-AR alpha 4 Antibody (S398A-34) [NBP2-59326] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-A Receptor Alpha 4 Monoclonal Antibody, Clone S398A-34 (NBP2-59326). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GABA-A Receptor Alpha 4 Monoclonal Antibody (NBP2-59326) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-A Receptor Alpha 4 Antibody (D) Composite.
Immunocytochemistry/ Immunofluorescence: GABA-AR alpha 4 Antibody (S398A-34) [NBP2-59326]

Immunocytochemistry/ Immunofluorescence: GABA-AR alpha 4 Antibody (S398A-34) [NBP2-59326]

Immunocytochemistry/Immunofluorescence: GABA-AR alpha 4 Antibody (S398A-34) [NBP2-59326] - Analysis using Mouse Anti-GABA-A Receptor Alpha4 Monoclonal Antibody, Clone S398A-34. (Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GABA-A Receptor Alpha4 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT.

Applications for GABA-AR alpha 4 Antibody (S398A-34) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: GABA-A R alpha 4

GABA (A) receptors are ligand-gated chloride channels that play a role as inhibitory neurotransmitters. They are known targets for certain classes of environmental and pharmaceutical compounds because a number of drugs interact with binding sites on GABA (A). Some of these drugs include benzodiazepines, anticonvulsants, and anesthetics. GABA (A) Receptors are comprised of subunits and these are further classified into 3 major groups (alpha, beta, and gamma). The subunits determine the pharmacological characteristics.

Long Name

gamma-Aminobutyric Acid Type A Receptor, alpha-4 Polypeptide

Alternate Names

GABAAR alpha 4, GABAARa4, GABRA4

Gene Symbol

GABRA4

Additional GABA-A R alpha 4 Products

Product Documents for GABA-AR alpha 4 Antibody (S398A-34) - BSA Free

Certificate of Analysis

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Product Specific Notices for GABA-AR alpha 4 Antibody (S398A-34) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

GABA Receptors

Citations for GABA-AR alpha 4 Antibody (S398A-34) - BSA Free

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Protocols

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