GABA-AR alpha 5 Antibody (JB34-19)

Novus Biologicals | Catalog # NBP2-75497

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JB34-19 expressed in HEK293
View all GABA-A R alpha 5 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein within Human GABA-AR alpha 5 aa 1-240 / 462. (SwissProt: P31644 Human; SwissProt: Q8BHJ7 Mouse; SwissProt: P19969 Rat)

Localization

Plasma membrane.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GABA-AR alpha 5 Antibody (JB34-19)

Western Blot: GABA-AR alpha 5 Antibody (JB34-19) [NBP2-75497]

Western Blot: GABA-AR alpha 5 Antibody (JB34-19) [NBP2-75497]

Western Blot: GABA-AR alpha 5 Antibody (JB34-19) [NBP2-75497] - Analysis of GABA A Receptor alpha 5 on different lysates using anti-GABA A Receptor alpha 5 antibody at 1/1,000 dilution. Positive control: Lane 1: A549 cell lysate Lane 2: 293T cell lysate Lane 3: Rat brain tissue lysate
Immunocytochemistry/ Immunofluorescence: GABA-AR alpha 5 Antibody (JB34-19) [NBP2-75497]

Immunocytochemistry/ Immunofluorescence: GABA-AR alpha 5 Antibody (JB34-19) [NBP2-75497]

Immunocytochemistry/Immunofluorescence: GABA-AR alpha 5 Antibody (JB34-19) [NBP2-75497] - Staining GABA A Receptor alpha 5 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: GABA-AR alpha 5 Antibody (JB34-19) [NBP2-75497]

Immunohistochemistry-Paraffin: GABA-AR alpha 5 Antibody (JB34-19) [NBP2-75497]

Immunohistochemistry-Paraffin: GABA-AR alpha 5 Antibody (JB34-19) [NBP2-75497] - Analysis of paraffin-embedded mouse brain tissue using anti-GABA A Receptor alpha 5 antibody. Counter stained with hematoxylin.
Immunocytochemistry/ Immunofluorescence: GABA-AR alpha 5 Antibody (JB34-19) [NBP2-75497]

Immunocytochemistry/ Immunofluorescence: GABA-AR alpha 5 Antibody (JB34-19) [NBP2-75497]

Immunocytochemistry/Immunofluorescence: GABA-AR alpha 5 Antibody (JB34-19) [NBP2-75497] - Staining GABA A Receptor alpha 5 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/ Immunofluorescence: GABA-AR alpha 5 Antibody (JB34-19) [NBP2-75497]

Immunocytochemistry/ Immunofluorescence: GABA-AR alpha 5 Antibody (JB34-19) [NBP2-75497]

Immunocytochemistry/Immunofluorescence: GABA-AR alpha 5 Antibody (JB34-19) [NBP2-75497] - Staining GABA A Receptor alpha 5 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: GABA-AR alpha 5 Antibody (JB34-19) [NBP2-75497]

Immunohistochemistry-Paraffin: GABA-AR alpha 5 Antibody (JB34-19) [NBP2-75497]

Immunohistochemistry-Paraffin: GABA-AR alpha 5 Antibody (JB34-19) [NBP2-75497] - Analysis of paraffin-embedded rat brain tissue using anti-GABA A Receptor alpha 5 antibody. Counter stained with hematoxylin.

Applications for GABA-AR alpha 5 Antibody (JB34-19)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GABA-A R alpha 5

GABA (A) receptors are ligand-gated chloride channels that play a role as inhibitory neurotransmitters. They are known targets for certain classes of environmental and pharmaceutical compounds because a number of drugs interact with binding sites on GABA (A). Some of these drugs include benzodiazepines, anticonvulsants, and anesthetics. GABA (A) Receptors are comprised of subunits and these are further classified into 3 major groups (alpha, beta, and gamma). The subunits determine the pharmacological characteristics.

Long Name

gamma-Aminobutyric Acid Type A Receptor, alpha-5 Polypeptide

Alternate Names

GABAAR alpha 5, GABAARa5, GABRA5

Gene Symbol

GABRA5

Additional GABA-A R alpha 5 Products

Product Documents for GABA-AR alpha 5 Antibody (JB34-19)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for GABA-AR alpha 5 Antibody (JB34-19)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

GABA Receptors

Citations for GABA-AR alpha 5 Antibody (JB34-19)

Customer Reviews for GABA-AR alpha 5 Antibody (JB34-19)

There are currently no reviews for this product. Be the first to review GABA-AR alpha 5 Antibody (JB34-19) and earn rewards!

Have you used GABA-AR alpha 5 Antibody (JB34-19)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies