GABPA Antibody (8C1B10)

Novus Biologicals | Catalog # NBP1-47416

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 8C1B10
View all GABPA Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human GABPA (aa120-190) expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GABPA Antibody (8C1B10)

Western Blot: GABPA Antibody (8C1B10)BSA Free [NBP1-47416]

Western Blot: GABPA Antibody (8C1B10)BSA Free [NBP1-47416]

Western Blot: GABPA Antibody (8C1B10) [NBP1-47416] - Analysis using GABPA mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY GABPA cDNA (2).
Immunocytochemistry/ Immunofluorescence: GABPA Antibody (8C1B10) - BSA Free [NBP1-47416]

Immunocytochemistry/ Immunofluorescence: GABPA Antibody (8C1B10) - BSA Free [NBP1-47416]

Immunocytochemistry/Immunofluorescence: GABPA Antibody (8C1B10) [NBP1-47416] - Analysis of Hela cells using GABPA mouse mAb (green). Red: Actin filaments have been labeled using DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Western Blot: GABPA Antibody (8C1B10)BSA Free [NBP1-47416]

Western Blot: GABPA Antibody (8C1B10)BSA Free [NBP1-47416]

Western Blot: GABPA Antibody (8C1B10) [NBP1-47416] - Analysis using GABPA mouse mAb against Hela (1), A549 (2), MCF-7 (3), NIH/3T3 (4) and SMMC-7721 (5) cell lysate.

Applications for GABPA Antibody (8C1B10)

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GABPA

This gene encodes one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit. Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, this gene may play a role in the Down Syndrome phenotype.

Alternate Names

E4TF1-60, E4TF1ATranscription factor E4TF1-60, GA binding protein transcription factor, alpha subunit 60kDa, GA-binding protein alpha chain, GA-binding protein transcription factor, alpha subunit (60kD), GABP subunit alpha, human nuclear respiratory factor-2 subunit alpha, 10, NFT2, NRF2, NRF2A, nuclear respiratory factor 2 alpha subunit, Nuclear respiratory factor 2 subunit alpha

Entrez Gene IDs

2551 (Human)

Gene Symbol

GABPA

Additional GABPA Products

Product Documents for GABPA Antibody (8C1B10)

Certificate of Analysis

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Product Specific Notices for GABPA Antibody (8C1B10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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