GABPA Antibody (M1) - Azide and BSA Free

Novus Biologicals | Catalog # H00002551-M03

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # M1

Format

Azide and BSA Free
View all GABPA Primary Antibodies »

Product Specifications

Immunogen

GABPA (AAH35031, 1 a.a. ~ 454 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTKREAEELIEIEIDGTEKAECTEESIVEQTYAPAECVSQAIDINEPIGNLKKLLEPRLQCSLDAHEICLQDIQLDPERSLFDQGVKTDGTVQLSVQVISYQGIEPKLNILEIVKPADTVEVVIDPDAHHAESEAHLVEEAQVITLDGTKHITTISDETSEQVTRWAAALEGYRKEQERLGIPYDPIQWSTDQVLHWVVWVMKEFSMTDIDLTTLNISGRELCSLNQEDFFQRVPRGEILWSHLELLRKYVLASQEQQMNEIVTIDQPVQIIPASVQSATPTTIKVINSSAKAAKVQRAPRISGEDRSSPGNRTGNNGQIQLWQFLLELLTDKDARDCISWVGDEGEFKLNQPELVAQKWGQRKNKPTMNYEKLSRALRYYYDGDMICKVQGKRFVYKFVCDLKTLIGYSAAELNRLVTECEQKKLAKMQLHGIAQPVTAVALSTASLQTEKDN

Specificity

GABPA - GA binding protein transcription factor, alpha subunit 60kDa

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for GABPA Antibody (M1) - Azide and BSA Free

Western Blot: GABPA Antibody (M1) [H00002551-M03]

Western Blot: GABPA Antibody (M1) [H00002551-M03]

Western Blot: GABPA Antibody (M1) [H00002551-M03] - Using 15 ug of human glioblastoma stem cells lysates. Primary antibody: GABPA dilution at 1:500 in 2.5% BSA/TBST. This image was submitted via customer Review.
Immunocytochemistry/ Immunofluorescence: GABPA Antibody (M1) [H00002551-M03]

Immunocytochemistry/ Immunofluorescence: GABPA Antibody (M1) [H00002551-M03]

Immunocytochemistry/Immunofluorescence: GABPA Antibody (M1) [H00002551-M03] - Analysis of monoclonal antibody to GABPA on HeLa cell. Antibody concentration 10 ug/ml
Western Blot: GABPA Antibody (M1) [H00002551-M03]

Western Blot: GABPA Antibody (M1) [H00002551-M03]

Western Blot: GABPA Antibody (M1) [H00002551-M03] - Analysis of GABPA expression in Hela S3 NE (Cat # L013V3).
Western Blot: GABPA Antibody (M1) [H00002551-M03]

Western Blot: GABPA Antibody (M1) [H00002551-M03]

Western Blot: GABPA Antibody (M1) [H00002551-M03] - Analysis of GABPA expression in transfected 293T cell line by GABPA monoclonal antibody (M03), clone M1. Lane 1: GABPA transfected lysate (Predicted MW: 51.3 KDa). Lane 2: Non-transfected lysate.
ELISA: GABPA Antibody (M1) [H00002551-M03]

ELISA: GABPA Antibody (M1) [H00002551-M03]

ELISA: GABPA Antibody (M1) [H00002551-M03] - Detection limit for recombinant GST tagged GABPA is approximately 1ng/ml as a capture antibody.
Immunoprecipitation: GABPA Antibody (M1) [H00002551-M03]

Immunoprecipitation: GABPA Antibody (M1) [H00002551-M03]

Immunoprecipitation: GABPA Antibody (M1) [H00002551-M03] - Analysis of GABPA transfected lysate using anti-GABPA monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with GABPA monoclonal antibody.

Applications for GABPA Antibody (M1) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Reviewed Applications

Read 1 review rated 3 using H00002551-M03 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GABPA

This gene encodes one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit. Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, this gene may play a role in the Down Syndrome phenotype.

Alternate Names

E4TF1-60, E4TF1ATranscription factor E4TF1-60, GA binding protein transcription factor, alpha subunit 60kDa, GA-binding protein alpha chain, GA-binding protein transcription factor, alpha subunit (60kD), GABP subunit alpha, human nuclear respiratory factor-2 subunit alpha, 10, NFT2, NRF2, NRF2A, nuclear respiratory factor 2 alpha subunit, Nuclear respiratory factor 2 subunit alpha

Entrez Gene IDs

2551 (Human)

Gene Symbol

GABPA

OMIM

600609 (Human)

UniProt

AAH35031 (Human)

Additional GABPA Products

Product Documents for GABPA Antibody (M1) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for GABPA Antibody (M1) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for GABPA Antibody (M1) - Azide and BSA Free (1)

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  • GABPA Antibody (M1)
    Name: Tanwarat Sanvoranart
    Application: Western Blot
    Sample Tested: human glioblastoma
    Species: Human
    Verified Customer | Posted 05/02/2017
    Using 15 ug of human glioblastoma stem cells lysates.
    SDS-PAGE in 10% polyacrylamide gel at constant 110 volts for 120 min. Blotting onto PVDF membrane at constant 300 mAmp 90 min at 4C. Primary antibody: GABPA dilution at 1:500 in 2.5% BSA/TBST, incubate at 4C overnight. Secondary antibody: anti-mouse IgG HRP conjugated dilution at 1:5000 in 2.5% BSA/TBST, incubate at RT for 1 hour.
    GABPA Antibody (M1) - Azide and BSA Free H00002551-M03

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FAQs

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