GABPA Antibody

Novus Biologicals | Catalog # NBP1-32105

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (99%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all GABPA Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human GABPA. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (80%), Chicken (88%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GABPA Antibody

Immunocytochemistry/ Immunofluorescence: GABPA Antibody [NBP1-32105]

Immunocytochemistry/ Immunofluorescence: GABPA Antibody [NBP1-32105]

Immunocytochemistry/Immunofluorescence: GABPA Antibody [NBP1-32105] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GABPA protein stained by GABPA antibody [N2C2], Internal diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining.Scale bar = 10 um.
GABPA Antibody

Western Blot: Rabbit Polyclonal GABPA Antibody [NBP1-32105] -

Western Blot: Rabbit Polyclonal GABPA Antibody [NBP1-32105] - Primary rat neonatal ventricular cardiomyocytes lysates with siRNA targeting Gabpa (siGabpa) and control (siCont). After 5 days of transfection with siRNA targeting rat Gabpa, the cells were lysed and subject to immunoblot analysis. Primary antibody dilution: 1/5000. Image from verified customer review.
Immunohistochemistry-Paraffin: GABPA Antibody [NBP1-32105]

Immunohistochemistry-Paraffin: GABPA Antibody [NBP1-32105]

Immunohistochemistry-Paraffin: GABPA Antibody [NBP1-32105] - Rat spleen. GABPA stained by GABPA antibody [N2C2], Internal diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunoprecipitation: GABPA Antibody [NBP1-32105]

Immunoprecipitation: GABPA Antibody [NBP1-32105]

Immunoprecipitation: GABPA Antibody [NBP1-32105] - Immunoprecipitation of GABPA protein from 293T whole cell extracts using 5 ug of GABPA antibody [N2C2], Internal. Western blot analysis was performed using GABPA antibody [N2C2], Internal. EasyBlot anti-Rabbit IgG was used as a secondary reagent.
GABPA Antibody [NBP1-32105] - Mouse lymph node. GABPA stained by GABPA antibody [N2C2], Internal diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
GABPA Antibody

Western Blot: GABPA Antibody [NBP1-32105] -

Western Blot: GABPA Antibody [NBP1-32105] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GABPA antibody [N2C2], Internal (NBP1-32105) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for GABPA Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunoprecipitation

1:100-1:500

Knockdown Validated

Validated for Knockdown from a verified customer review.

Western Blot

1:500-1:3000
Application Notes
IHC-P-Assay dependent

Reviewed Applications

Read 1 review rated 5 using NBP1-32105 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GABPA

This gene encodes one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit. Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, this gene may play a role in the Down Syndrome phenotype.

Alternate Names

E4TF1-60, E4TF1ATranscription factor E4TF1-60, GA binding protein transcription factor, alpha subunit 60kDa, GA-binding protein alpha chain, GA-binding protein transcription factor, alpha subunit (60kD), GABP subunit alpha, human nuclear respiratory factor-2 subunit alpha, 10, NFT2, NRF2, NRF2A, nuclear respiratory factor 2 alpha subunit, Nuclear respiratory factor 2 subunit alpha

Gene Symbol

GABPA

Additional GABPA Products

Product Documents for GABPA Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for GABPA Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for GABPA Antibody (1)

5 out of 5
1 Customer Rating
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Customer Images

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  • GABPA Antibody
    Name: Shinichi Oka
    Application: Western Blot
    Sample Tested: primary neonatal ventricular cardiomyocytes
    Species: Rat
    Verified Customer | Posted 08/29/2023
    Primary rat neonatal ventricular cardiomyocytes lysates with siRNA targeting Gabpa (siGabpa) and control (siCont).
    After 5 days of transfection with siRNA targeting rat Gabpa, the cells were lysed and subject to immunoblot analysis. Experimental conditions of immunoblot: Membrane: PDVF Blocking: 5% BSA/TBS-T for 0.5 hour First antibody (NBP1-32105) 1/5000 dilution (1 microL/5ml TBS-T) for overnight (16 hours) at 4 degrees. Second antibody HRP-anti-Rabbit IgG (Cell Signaling Technology 7074) 1/5000 dilution (1 microL/5ml TBS-T) for 1 hour at room temperature.
    GABPA Antibody NBP1-32105

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Protocols

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FAQs

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