GABPA Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84942

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GABPA Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EELIEIEIDGTEKAECTEESIVEQTYAPAECVSQAIDINEPIGNLKKLLEPRLQCSLDAHEICLQDIQLDPERSLFDQGVKTDGTVQLSVQVISYQGIEPKLNILEIVKPADTVEVVIDPDAHHAESEAHLVEEAQVITLDGTK

Reactivity Notes

Reactivity reported in scientific literature (PMID: 24753418)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GABPA Antibody - BSA Free

Western Blot: GABPA Antibody [NBP1-84942]

Western Blot: GABPA Antibody [NBP1-84942]

Western Blot: GABPA Antibody [NBP1-84942] - Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human liver tissue. Lane 5: Human tonsil tissue
Immunocytochemistry/ Immunofluorescence: GABPA Antibody [NBP1-84942]

Immunocytochemistry/ Immunofluorescence: GABPA Antibody [NBP1-84942]

Immunocytochemistry/Immunofluorescence: GABPA Antibody [NBP1-84942] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: GABPA Antibody [NBP1-84942]

Immunohistochemistry-Paraffin: GABPA Antibody [NBP1-84942]

Immunohistochemistry-Paraffin: GABPA Antibody [NBP1-84942] - Staining of human lymph node shows strong nuclear positivity in non-germinal center cells.
GABPA Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: GABPA Antibody - BSA Free [NBP1-84942]

Chromatin Immunoprecipitation-exo-Seq: GABPA Antibody - BSA Free [NBP1-84942]

ChIP-Exo-Seq composite graph for Anti-GABPA (NBP1-84942) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for GABPA Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GABPA

GABPA encodes one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit. Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, this gene may play a role in the Down Syndrome phenotype.

Alternate Names

E4TF1-60, E4TF1ATranscription factor E4TF1-60, GA binding protein transcription factor, alpha subunit 60kDa, GA-binding protein alpha chain, GA-binding protein transcription factor, alpha subunit (60kD), GABP subunit alpha, human nuclear respiratory factor-2 subunit alpha, 10, NFT2, NRF2, NRF2A, nuclear respiratory factor 2 alpha subunit, Nuclear respiratory factor 2 subunit alpha

Gene Symbol

GABPA

Additional GABPA Products

Product Documents for GABPA Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for GABPA Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GABPA Antibody - BSA Free

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Protocols

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FAQs

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