GAD2/GAD65 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89801

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GAD2/GAD65 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SGFWSFGSEDGSGDSENPGTARAWCQVAQKFTGGIGNKLCALLYGDAEKPAESGGSQPPRAAARKAACACDQKPCSCSKVDVNYAFLHATDLLP

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%), Rat (88%)

Marker

GABAergic Neuronal Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GAD2/GAD65 Antibody - BSA Free

Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody [NBP1-89801]

Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody [NBP1-89801]

Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody [NBP1-89801] - Staining of human skeletal muscle shows no granular cytoplasmic positivity in myocytes as expected.
Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody [NBP1-89801]

Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody [NBP1-89801]

Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody [NBP1-89801] - Staining of human cerebellum shows moderate granular cytoplasmic positivity in cells in molecular layer.
Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody [NBP1-89801]

Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody [NBP1-89801]

Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody [NBP1-89801] - Staining of human cerebral cortex shows moderate granular cytoplasmic positivity in neuropil.
Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody [NBP1-89801]

Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody [NBP1-89801]

Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody [NBP1-89801] - Staining of human pancreas shows strong granular cytoplasmic positivity in islets of Langerhans.

Applications for GAD2/GAD65 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GAD2/GAD65

Antibodies to glutamic acid decarboxylase-65 (GAD65) are present in autoimmune disorders such as insulin-dependent (type 1) diabetes mellitus (IDDM), stiff man syndrome and polyendocrine autoimmune disease. Auto-antibodies to GAD65 are present in 60-70% of individuals with newly diagnosed IDDM and thus are important markers for disease activity. These auto-antibodies usually recognize conformation-dependent regions on GAD65 and rarely bind to the second isoform, GAD67. Auto-antibodies to GAD67 are found in only 15% of recent-onset IDDM patients and most of this binding can be blocked with GAD65, suggesting shared epitopes between the two isoforms of GAD.

Long Name

Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa)

Alternate Names

GAD65

Gene Symbol

GAD2

Additional GAD2/GAD65 Products

Product Documents for GAD2/GAD65 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for GAD2/GAD65 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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