GADD45 alpha Antibody (3D12) - Azide and BSA Free

Novus Biologicals | Catalog # H00001647-M01

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Simple Western, Proximity Ligation Assay

Cited:

Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3D12

Format

Azide and BSA Free
View all GADD45 alpha Primary Antibodies »

Product Specifications

Immunogen

GADD45A (AAH11757, 76 a.a. ~ 165 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TLIQAFCCENDINILRVSNPGRLAELLLLETDAGPAASEGAEQPPDLHCVLVTNPHSSQWKDPALSQLICFCRESRYMDQWVPVINLPER

Specificity

GADD45A - growth arrest and DNA-damage-inducible, alpha

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for GADD45 alpha Antibody (3D12) - Azide and BSA Free

Proximity Ligation Assay: GADD45 alpha Antibody (3D12) [H00001647-M01]

Proximity Ligation Assay: GADD45 alpha Antibody (3D12) [H00001647-M01]

Proximity Ligation Assay: GADD45 alpha Antibody (3D12) [H00001647-M01] - Analysis of protein-protein interactions between MAP2K6 and GADD45A. HeLa cells were stained with anti-MAP2K6 rabbit purified polyclonal 1:1200 and anti-GADD45A mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Applications for GADD45 alpha Antibody (3D12) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.Use in Simple Western reported in scientific literature (PMID:29890265).
See Simple Western Antibody Database for Simple Western validation: separated by Size

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GADD45 alpha

This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms.

Long Name

Growth Arrest and DNA Damage Gene alpha

Alternate Names

GADD45a

Entrez Gene IDs

1647 (Human)

Gene Symbol

GADD45A

OMIM

126335 (Human)

UniProt

AAH11757 (Human)

Additional GADD45 alpha Products

Product Documents for GADD45 alpha Antibody (3D12) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for GADD45 alpha Antibody (3D12) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Adult Neurogenesis
Cancer Biomarkers

Citations for GADD45 alpha Antibody (3D12) - Azide and BSA Free

Customer Reviews for GADD45 alpha Antibody (3D12) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review GADD45 alpha Antibody (3D12) - Azide and BSA Free and earn rewards!

Have you used GADD45 alpha Antibody (3D12) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies