GALE Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87066

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GALE Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QGIPNNLMPYVSQVAIGRREALNVFGNDYDTEDGTGVRDYIHVVDLAKGHIAALRKLKEQCGCRIYNLGTGTGYSVLQMVQAMEKASGKKIPYKVVARREGDVAA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GALE Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: GALE Antibody [NBP1-87066]

Immunocytochemistry/ Immunofluorescence: GALE Antibody [NBP1-87066]

Immunocytochemistry/Immunofluorescence: GALE Antibody [NBP1-87066] - Staining of human cell line A-431 shows localization to nucleus, plasma membrane & cytosol.
Immunohistochemistry-Paraffin: GALE Antibody [NBP1-87066]

Immunohistochemistry-Paraffin: GALE Antibody [NBP1-87066]

Immunohistochemistry-Paraffin: GALE Antibody [NBP1-87066] - Staining of human colon shows strong cytoplasmic and nuclear positivity in glandular cells.
GALE Antibody - BSA Free Western Blot: GALE Antibody - BSA Free [NBP1-87066]

Western Blot: GALE Antibody - BSA Free [NBP1-87066]

Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
GALE Antibody - BSA Free Western Blot: GALE Antibody - BSA Free [NBP1-87066]

Western Blot: GALE Antibody - BSA Free [NBP1-87066]

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp

Applications for GALE Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 1 review rated 4 using NBP1-87066 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GALE

GALE encodes UDP-galactose-4-epimerase which catalyzes two distinct but analogous reactions: the epimerization of UDP-glucose to UDP-galactose, and the epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The bifunctional nature of the enzyme has the important metabolic consequence that mutant cells (or individuals) are dependent not only on exogenous galactose, but also on exogenous N-acetylgalactosamine as a necessary precursor for the synthesis of glycoproteins and glycolipids. Mutations in this gene result in epimerase-deficiency galactosemia, also referred to as galactosemia type 3, a disease characterized by liver damage, early-onset cataracts, deafness and mental retardation, with symptoms ranging from mild ('peripheral' form) to severe ('generalized' form). Multiple alternatively spliced transcripts encoding the same protein have been identified.

Alternate Names

EC 5.1.3, EC 5.1.3.2, FLJ95174, FLJ97302, Galactowaldenase, short chain dehydrogenase/reductase family 1E, member 1, UDP-, UDP galactose-4'-epimerase, UDP-galactose 4-epimerase, UDP-galactose-4-epimerase, UDP-glucose 4-epimerase

Gene Symbol

GALE

Additional GALE Products

Product Documents for GALE Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for GALE Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GALE Antibody - BSA Free

Customer Reviews for GALE Antibody - BSA Free (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: hela whole cell lysate, primary hepatocyte lysate
    Species: Human
    Verified Customer | Posted 06/17/2015
    Gale Antibody against GALE overexpression lysate
    GALE Antibody - BSA Free NBP1-87066

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