Galectin-3 Antibody (5C21) - BSA Free

Novus Biologicals | Catalog # NBP1-92690

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5C21

Format

BSA Free
View all Galectin-3 Primary Antibodies »

Product Specifications

Immunogen

Full length recombinant human Galectin-3 expressed in and purified from E. coli. [UniProt# P17931]

Localization

Cytoplasm. Nucleus. Secreted. Note: Secreted by a non-classical secretory pathway and associates with the cell surface.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Galectin-3 Antibody (5C21) - BSA Free

Western Blot: Galectin-3 Antibody (5C21) [NBP1-92690]

Western Blot: Galectin-3 Antibody (5C21) [NBP1-92690]

Western Blot: Galectin-3 Antibody (5C21) [NBP1-92690] - Analysis of different tissue and cell lysates using mouse mAb to galectin-3, NBP1-92690, dilution 1:2,000 in green: [1] protein standard (red), [2] mouse brain lysate, rat tissue lysates: [3] brain, [4] liver, [5] heart, [6] kidney, [7] lung, and [8] HEK293 cell lysate. The band at about 30kDa mark corresponds to the galectin-3 protein. The same blot was simultaneously probed with chicken pAb to HSP60, dilution 1:20,000, in red, which reveals HSP60 protein in all preparations.
Immunocytochemistry/ Immunofluorescence: Galectin-3 Antibody (5C21) [NBP1-92690]

Immunocytochemistry/ Immunofluorescence: Galectin-3 Antibody (5C21) [NBP1-92690]

Immunocytochemistry/Immunofluorescence: Galectin-3 Antibody (5C21) [NBP1-92690] - MDA-MB-231 cells stained for Gal-3 (red, arrows), shown with DAPI counterstained nuclei (blue). Negative control received non-specific antibody instead of primary antibody. Image from a verified customer review.
Immunocytochemistry/ Immunofluorescence: Galectin-3 Antibody (5C21) [NBP1-92690]

Immunocytochemistry/ Immunofluorescence: Galectin-3 Antibody (5C21) [NBP1-92690]

Immunocytochemistry/Immunofluorescence: Galectin-3 Antibody (5C21) [NBP1-92690] - Analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to galectin-3, NBP1-92690, dilution 1:1,000 in red, and costained with chicken pAb to MAP2, dilution 1:10,000 in green. The blue is Hoechst staining of nuclear DNA. The NBP1-92690 antibody produces strong staining of microglia (top panel), other glial cells (bottom panel) and lymphocytic cells (small round cells), while the MAP2 antibody labels dendrites and perikarya of mature neurons.

Applications for Galectin-3 Antibody (5C21) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000

Immunohistochemistry

1:1000

Western Blot

1:2000
Application Notes
This Galectin-3 (5C21) antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry, and Western blot, where a band can be seen at approximately 30 kDa.

Reviewed Applications

Read 1 review rated 4 using NBP1-92690 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50% PBS, 50% glycerol

Format

BSA Free

Preservative

5mM Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Galectin-3

Galectin-3 (LGALS3 or Mac-2 antigen), is a 26 kD beta-galactoside-binding protein from galectin family and it is ubiquitously expressed with localization dependent on cell type as well as proliferative status. Galectin-3 is expressed in many immunocompetent and inflammatory cells, including macrophages, dendritic cells, eosinophils, mast cells, uterine NK cells and activated T and B cells, and it is localized in nucleus, cytoplasm or on cell surface as well as in extracellular compartments. Extracellularly, galectin-3 acts as an adhesion molecule whereas intracellular galectin-3 implicates in processes that are pivotal for basic cellular functions, such as pre-mRNA splicing regulation of cell growth, cell cycle progression and apoptosis. Galectin-3 binds and interacts with numerous ligands in intra-/extracellular environment such as DMBT1, ITGA3, ITGB1, CSPG4, LGALS3BP, LYPD3, CYHR1, UACA etc. for modulating cellular signals responsible fpr its biological functions such as phagocytosis, cell growth/proliferation, adhesion and apoptosis in normal as well as cancer cells. Circulating galectin-3 concentrations increases more than 30-fold in bloodstream of patients with various cancers and patients with metastatic disease have higher concentrations of circulating galectin-3 than those with only localized tumors.

Alternate Names

AGE-R3, CBP35, GAL3, Galectin3, L29, LGALS3, Mac-2

Entrez Gene IDs

3958 (Human)

Gene Symbol

LGALS3

UniProt

P17931 (Mouse)

Additional Galectin-3 Products

Product Documents for Galectin-3 Antibody (5C21) - BSA Free

Certificate of Analysis

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Product Specific Notices for Galectin-3 Antibody (5C21) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Galectin-3 Antibody (5C21) - BSA Free

Customer Reviews for Galectin-3 Antibody (5C21) - BSA Free (1)

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  • Galectin-3 Antibody (5C21)
    Name: Gail Seigel
    Application: Immunocytochemistry
    Sample Tested: Human MDA-MB-231 cells and Cytospin of cell culture cells
    Species: Human
    Verified Customer | Posted 07/17/2019
    MDA-MB-231 cells stained for Gal-3 (red, arrows), shown with DAPI counterstained nuclei (blue). Negative control received non-specific antibody instead of primary antibody.
    MDA-MB-231 cells were incubated with Gal-3 antibody (1:100 dilution) in PBS-Tween for one hour at room temperature.
    Galectin-3 Antibody (5C21) - BSA Free NBP1-92690

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