Galectin-3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-03252

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Knockout Validated, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Galectin-3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human Galectin 3/Galectin-3 (NP_002297.2). MADNFSLHDALSGSGNPNPQGWPGAWGNQPAGAGGYPGASYPGAYPGQAPPGAYPGQAPPGAYPGAPGAYPGAPAPGVYPGPPSGPGAYPSSGQPSATGAYPATGPYGAPAGPLIVPYNLPLPGGVVPRMLITILGTVKPNANRIALDFQRGNDVAFHFNPRFNENNRRVIVCNTKLDNNWGREERQSVFPFESGKPFKIQVLVEPDHFKVAVNDAHLLQYNHRVKKLNEISKLGISGDIDLTSASYTMI

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Knockout (KO) Validated Rabbit Galectin-3 Antibody - BSA Free (NBP3-03252) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Galectin-3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Galectin-3 Antibody - BSA Free

Knockout Validated: Galectin-3 Antibody - Azide and BSA Free [NBP3-03252]

Western Blot: Galectin-3 Antibody - Azide and BSA Free [NBP3-03252]

Western Blot: Galectin-3 Antibody [NBP3-03252] - Analysis of extracts from normal (control) and LGALS3 knockout (KO) HeLa cells, using Galectin-3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: nonfat dry milk in TBST.
Galectin-3 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Galectin-3 Antibody - Azide and BSA Free [Galectin-3] -

Immunocytochemistry/ Immunofluorescence: Galectin-3 Antibody - Azide and BSA Free [Galectin-3] - Immunofluorescence analysis of U-2 OS cells using [KO Validated] Galectin-3 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Galectin-3 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Galectin-3 Antibody - Azide and BSA Free [Galectin-3] -

Immunocytochemistry/ Immunofluorescence: Galectin-3 Antibody - Azide and BSA Free [Galectin-3] - Immunofluorescence analysis of C6 cells using [KO Validated] Galectin-3 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Galectin-3 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Galectin-3 Antibody - Azide and BSA Free [Galectin-3] -

Immunocytochemistry/ Immunofluorescence: Galectin-3 Antibody - Azide and BSA Free [Galectin-3] - Immunofluorescence analysis of L-929 cells using [KO Validated] Galectin-3 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for Galectin-3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Galectin-3

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis

Alternate Names

AGE-R3, CBP35, GAL3, Galectin3, L29, LGALS3, Mac-2

Gene Symbol

LGALS3

Additional Galectin-3 Products

Product Documents for Galectin-3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Galectin-3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Citations for Galectin-3 Antibody - BSA Free

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Protocols

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