GALNT2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32394

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human GALNT2. The exact sequence is proprietary.

Localization

Golgi apparatus, Golgi stack membrane, Single-pass type II membrane protein, Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GALNT2 Antibody - BSA Free

Western Blot: GALNT2 Antibody [NBP1-32394]

Western Blot: GALNT2 Antibody [NBP1-32394]

Western Blot: GALNT2 Antibody [NBP1-32394] - Sample (50 ug of whole cell lysate) A: Mouse white adipose tissue 7. 5% SDS PAGE; antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: GALNT2 Antibody [NBP1-32394]

Immunohistochemistry-Paraffin: GALNT2 Antibody [NBP1-32394]

Immunohistochemistry-Paraffin: GALNT2 Antibody [NBP1-32394] - Paraffin-embedded Colon ca, using antibody at 1:250 dilution.
GALNT2 Antibody

Immunocytochemistry/ Immunofluorescence: GALNT2 Antibody [NBP1-32394] -

Immunocytochemistry/ Immunofluorescence: GALNT2 Antibody [NBP1-32394] - GALNT2 antibody detects GALNT2 protein at Golgi apparatus by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: GALNT2 stained by GALNT2 antibody (NBP1-32394) diluted at 1:500.
Red: alpha Tubulin, stained by alpha Tubulin antibody [GT114] diluted at 1:500.
Blue: Fluoroshield with DAPI.
Scale bar= 10 um.
GALNT2 Antibody

Western Blot: GALNT2 Antibody [NBP1-32394] -

Western Blot: GALNT2 Antibody [NBP1-32394] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GALNT2 antibody (NBP1-32394) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
GALNT2 Antibody - BSA Free

Western Blot: GALNT2 Antibody [NBP1-32394] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GALNT2 antibody (NBP1-32394) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data are based on Human Protein Atlas program.
GALNT2 Antibody - BSA Free

Western Blot: GALNT2 Antibody [NBP1-32394] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GALNT2 antibody (NBP1-32394) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Applications for GALNT2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Polypeptide GalNAc Transferase 2/GALNT2

This gene encodes polypeptide N-acetylgalactosaminyltransferase 2, a member of the GalNAc-transferases family. This family transfers an N-acetyl galactosamine to the hydroxyl group of a serine or threonine residue in the first step of O-linked oligosaccharide biosynthesis. Individual GalNAc-transferases have distinct activities and initiation of O-glycosylation in a cell is regulated by a repertoire of GalNAc-transferases.

Long Name

UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 2

Alternate Names

GalNAc-T2, Polypeptide GalNac Transferase 2, pp-GaNTase 2

Gene Symbol

GALNT2

Additional Polypeptide GalNAc Transferase 2/GALNT2 Products

Product Documents for GALNT2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for GALNT2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

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Protocols

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