GALNT2 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-00188

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all Polypeptide GalNAc Transferase 2/GALNT2 Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human GALNT2 (Accession#: NP_004472.1; Lys52-Gln571)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for GALNT2 Antibody - Azide and BSA Free

GALNT2 Antibody - Azide and BSA Free

Immunohistochemistry-Paraffin: GALNT2 Antibody [NBP3-00188] -

Immunohistochemistry-Paraffin: GALNT2 Antibody [NBP3-00188] - Immunochemical staining of human GALNT2 in human brain with NBP3-00188 (1:1000, formalin-fixed paraffin embedded sections).
GALNT2 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: GALNT2 Antibody [NBP3-00188] -

Immunocytochemistry/ Immunofluorescence: GALNT2 Antibody [NBP3-00188] - Immunofluorescence staining of GALNT2 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-00188 (1:1000) at 4 overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
GALNT2 Antibody - Azide and BSA Free

Western Blot: GALNT2 Antibody [NBP3-00188] -

Western Blot: GALNT2 Antibody [NBP3-00188] - NBP3-00188 at 1:500 dilution Lane A: A549 Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lane C: U87MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:65 kDa Observed band size:65 kDa (We are unsure as to the identity of these extra bands.)
GALNT2 Antibody - Azide and BSA Free

Immunoprecipitation: GALNT2 Antibody [NBP3-00188] -

Immunoprecipitation: GALNT2 Antibody [NBP3-00188] - GALNT2 was immunoprecipitated using: Lane A:0.5 mg A549 Whole Cell Lysate Lane B:0.5 mg Hela Whole Cell Lysate Lane C:0.5 mg U87MG Whole Cell Lysate 4 uL NBP3-00188 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00188, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 71 kDa Observed band size: 71 kDa

Applications for GALNT2 Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Immunohistochemistry-Paraffin

1:500-1:2000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Polypeptide GalNAc Transferase 2/GALNT2

GALNT2 encodes polypeptide N-acetylgalactosaminyltransferase 2, a member of the GalNAc-transferases family. This family transfers an N-acetyl galactosamine to the hydroxyl group of a serine or threonine residue in the first step of O-linked oligosaccharide biosynthesis. Individual GalNAc-transferases have distinct activities and initiation of O-glycosylation in a cell is regulated by a repertoire of GalNAc-transferases.

Long Name

UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 2

Alternate Names

GalNAc-T2, Polypeptide GalNac Transferase 2, pp-GaNTase 2

Gene Symbol

GALNT2

Additional Polypeptide GalNAc Transferase 2/GALNT2 Products

Product Documents for GALNT2 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for GALNT2 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Glycobiology-related Enzymes

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Protocols

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FAQs

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