GALT Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-86138

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RANDHQHIRYNPLQDEWVLVSAHRMKRPWQGQVEPQLLKTVPRHDPLNPLCPGAIRANGEVNPQYDSTFLFDNDFPALQPDAPSPGPSDHPLFQAKSARGVCKVMCFHPWSD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GALT Antibody - BSA Free

GALT Antibody - BSA Free Immunohistochemistry-Paraffin: GALT Antibody - BSA Free [NBP1-86138]

Immunohistochemistry-Paraffin: GALT Antibody - BSA Free [NBP1-86138]

Staining of human prostate shows moderate cytoplasmic positivity in glandular cells.
GALT Antibody - BSA Free Immunohistochemistry-Paraffin: GALT Antibody - BSA Free [NBP1-86138]

Immunohistochemistry-Paraffin: GALT Antibody - BSA Free [NBP1-86138]

Staining of human liver shows strong cytoplasmic positivity in hepatocytes.
GALT Antibody - BSA Free Immunohistochemistry-Paraffin: GALT Antibody - BSA Free [NBP1-86138]

Immunohistochemistry-Paraffin: GALT Antibody - BSA Free [NBP1-86138]

Staining of human gastrointestinal, liver, placenta and prostate using Anti-GALT antibody NBP1-86138 (A) shows similar protein distribution across tissues to independent antibody NBP1-86133 (B).
GALT Antibody - BSA Free Immunohistochemistry-Paraffin: GALT Antibody - BSA Free [NBP1-86138]

Immunohistochemistry-Paraffin: GALT Antibody - BSA Free [NBP1-86138]

Staining of human gastrointestinal shows strong cytoplasmic positivity in glandular cells.
GALT Antibody - BSA Free Immunohistochemistry-Paraffin: GALT Antibody - BSA Free [NBP1-86138]

Immunohistochemistry-Paraffin: GALT Antibody - BSA Free [NBP1-86138]

Staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.
GALT Antibody - BSA Free Western Blot: GALT Antibody - BSA Free [NBP1-86138]

Western Blot: GALT Antibody - BSA Free [NBP1-86138]

Analysis in human liver tissue.

Applications for GALT Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GALT

Galactose-1-phosphate uridyl transferase (GALT) catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of UDP-glucose + galactose-1-phosphate to glucose-1-phosphate + UDP-galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet. The pathophysiology of galactosemia has not been clearly defined.

Long Name

galactose-1-phosphate uridylyltransferase

Alternate Names

EC 2.7.7.12, Gal-1-P uridylyltransferase, galactose-1-phosphate uridyl transferase, galactose-1-phosphate uridylyltransferase, UDP-glucose--hexose-1-phosphate uridylyltransferase

Gene Symbol

GALT

Additional GALT Products

Product Documents for GALT Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for GALT Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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