gamma Catenin Antibody (15F11) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-54527
![Western Blot: gamma Catenin Antibody (15F11)Azide and BSA Free [NBP2-54527]](https://resources.rndsystems.com/images/products/gamma-Catenin-Antibody-15F11-Azide-and-BSA-Free-Western-Blot-NBP2-54527-img0003.jpg "Western Blot: gamma Catenin Antibody (15F11)Azide and BSA Free [NBP2-54527]")
Key Product Details
Species Reactivity
Human, Mouse, Rat, Bovine, Canine, Chicken, Feline
Applications
Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 15F11
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant, full length chicken gamma Catenin protein (Uniprot: P14923)
Localization
Cell Surface and Cytoplasmic
Marker
Cardiomyocyte Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53325).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for gamma Catenin Antibody (15F11) - Azide and BSA Free
Western Blot: gamma Catenin Antibody (15F11)Azide and BSA Free [NBP2-54527]
Western Blot: gamma Catenin Antibody (15F11) - Azide and BSA Free [NBP2-54527] - Western Blot Analysis of human HeLa and SK-BR3 cell lysate using gamma Catenin Antibody (15F11).![Immunocytochemistry/ Immunofluorescence: gamma Catenin Antibody (15F11) - Azide and BSA Free [NBP2-54527]](https://resources.rndsystems.com/images/products/gamma-Catenin-Antibody-15F11-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-54527-img0001.jpg "Immunocytochemistry/ Immunofluorescence: gamma Catenin Antibody (15F11) - Azide and BSA Free [NBP2-54527]")
Immunocytochemistry/ Immunofluorescence: gamma Catenin Antibody (15F11) - Azide and BSA Free [NBP2-54527]
Immunocytochemistry/Immunofluorescence: gamma Catenin Antibody (15F11) - Azide and BSA Free [NBP2-54527] - Confocal immunofluorescence image of HeLa cells using gamma Catenin Antibody (15F11) Green (CF488) and Red Dot is used to label the nuclei Red.![Immunohistochemistry-Paraffin: gamma Catenin Antibody (15F11) - Azide and BSA Free [NBP2-54527]](https://resources.rndsystems.com/images/products/gamma-Catenin-Antibody-15F11-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54527-img0005.jpg "Immunohistochemistry-Paraffin: gamma Catenin Antibody (15F11) - Azide and BSA Free [NBP2-54527]")
Immunohistochemistry-Paraffin: gamma Catenin Antibody (15F11) - Azide and BSA Free [NBP2-54527]
Immunohistochemistry-Paraffin: gamma Catenin Antibody (15F11) - Azide and BSA Free [NBP2-54527] - Formalin-fixed, paraffin-embedded human cervix stained with gamma Catenin Antibody (15F11). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.![Flow Cytometry: gamma Catenin Antibody (15F11) - Azide and BSA Free [NBP2-54527]](https://resources.rndsystems.com/images/products/gamma-Catenin-Antibody-15F11-Azide-and-BSA-Free-Flow-Cytometry-NBP2-54527-img0004.jpg "Flow Cytometry: gamma Catenin Antibody (15F11) - Azide and BSA Free [NBP2-54527]")
Flow Cytometry: gamma Catenin Antibody (15F11) - Azide and BSA Free [NBP2-54527]
Flow Cytometry: gamma Catenin Antibody (15F11) - Azide and BSA Free [NBP2-54527] - Flow Cytometric Analysis of PFA-fixed MCF cells. gamma Catenin Antibody (15F11) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).![Western Blot: gamma Catenin Antibody (15F11)Azide and BSA Free [NBP2-54527]](https://resources.rndsystems.com/images/products/gamma-Catenin-Antibody-15F11-Azide-and-BSA-Free-Western-Blot-NBP2-54527-img0002.jpg "Western Blot: gamma Catenin Antibody (15F11)Azide and BSA Free [NBP2-54527]")
Western Blot: gamma Catenin Antibody (15F11)Azide and BSA Free [NBP2-54527]
Western Blot: gamma Catenin Antibody (15F11) - Azide and BSA Free [NBP2-54527] - Analysis of HT20, 293, A431, MCF-7, HepG2 and A549 cell lysates using Catenin, gamma Mouse Monoclonal Antibody (15F11)
gamma Catenin Antibody (15F11)
Formalin-fixed, paraffin-embedded human cervix stained with gamma Catenin antibody (15F11). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.Applications for gamma Catenin Antibody (15F11) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5 - 1 ug/million cells
Immunocytochemistry/ Immunofluorescence
1 - 2 ug/ml
Western Blot
0.5 - 1.0 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: gamma Catenin
Alternate Names
catenin (cadherin-associated protein), gamma (80kD), catenin (cadherin-associated protein), gamma 80kDa, Catenin gamma, Desmoplakin III, desmoplakin-3, DP3CTNNG, DPIII, gamma-catenin, junction plakoglobin, PDGBARVD12, PKGB
Gene Symbol
JUP
Additional gamma Catenin Products
Product Documents for gamma Catenin Antibody (15F11) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for gamma Catenin Antibody (15F11) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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