gamma Catenin Antibody (15F11)

Novus Biologicals | Catalog # NBP2-53325

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine, Canine, Chicken, Feline

Applications

Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 15F11
View all gamma Catenin Primary Antibodies »

Product Specifications

Immunogen

Recombinant, full length chicken gamma Catenin protein (Uniprot: P14923)

Localization

Cell Surface and Cytoplasmic

Marker

Cardiomyocyte Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54527)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for gamma Catenin Antibody (15F11)

Western Blot: gamma Catenin Antibody (15F11) [NBP2-53325]

Western Blot: gamma Catenin Antibody (15F11) [NBP2-53325]

Western Blot: gamma Catenin Antibody (15F11) [NBP2-53325] - Western Blot Analysis of human HeLa and SK-BR3 cell lysate using gamma Catenin Antibody (15F11).
Immunocytochemistry/ Immunofluorescence: gamma Catenin Antibody (15F11) [NBP2-53325]

Immunocytochemistry/ Immunofluorescence: gamma Catenin Antibody (15F11) [NBP2-53325]

Immunocytochemistry/Immunofluorescence: gamma Catenin Antibody (15F11) [NBP2-53325] - Confocal immunofluorescence image of HeLa cells using gamma Catenin Antibody (15F11) Green (CF488) and Red Dot is used to label the nuclei Red.
Immunohistochemistry-Paraffin: gamma Catenin Antibody (15F11) [NBP2-53325]

Immunohistochemistry-Paraffin: gamma Catenin Antibody (15F11) [NBP2-53325]

Immunohistochemistry-Paraffin: gamma Catenin Antibody (15F11) [NBP2-53325] - Formalin-fixed, paraffin-embedded human cervix stained with gamma Catenin Antibody (15F11). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.
Flow Cytometry: gamma Catenin Antibody (15F11) [NBP2-53325]

Flow Cytometry: gamma Catenin Antibody (15F11) [NBP2-53325]

Flow Cytometry: gamma Catenin Antibody (15F11) [NBP2-53325] - Flow Cytometric Analysis of PFA-fixed MCF cells. gamma Catenin Antibody (15F11) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Western Blot: gamma Catenin Antibody (15F11) [NBP2-53325]

Western Blot: gamma Catenin Antibody (15F11) [NBP2-53325]

Western Blot: gamma Catenin Antibody (15F11) [NBP2-53325] - Analysis of HT20, 293, A431, MCF-7, HepG2 and A549 cell lysates using Catenin, gamma Mouse Monoclonal Antibody (15F11)

Applications for gamma Catenin Antibody (15F11)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: gamma Catenin

Catenin gamma, also known as Junction Plakoglobin, is a common junctional plaque protein. It is a cytoplasmic protein with soluble and membrane-associated forms. The presence of Catenin gamma in both the desmosomes and in the intermediate junctions suggests it has a role in the structure and Function of submembranous plaques.

Alternate Names

catenin (cadherin-associated protein), gamma (80kD), catenin (cadherin-associated protein), gamma 80kDa, Catenin gamma, Desmoplakin III, desmoplakin-3, DP3CTNNG, DPIII, gamma-catenin, junction plakoglobin, PDGBARVD12, PKGB

Gene Symbol

JUP

UniProt

P14923 (Human)

Additional gamma Catenin Products

Product Documents for gamma Catenin Antibody (15F11)

Certificate of Analysis

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Product Specific Notices for gamma Catenin Antibody (15F11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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