gamma Sarcoglycan Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-90299

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all gamma-Sarcoglycan/SGCG Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: YAKEIHSRVDSSLLLQSTQNVTVNARNSEGEVTGRLKVGPKMVEVQNQQFQINSNDGKPLFTVDEKEVVVGTDKLRVTGPEGALFEHSVETPLVRADPFQD

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (83%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for gamma Sarcoglycan Antibody - BSA Free

gamma Sarcoglycan Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: gamma Sarcoglycan Antibody - BSA Free [NBP1-90299]

Immunocytochemistry/ Immunofluorescence: gamma Sarcoglycan Antibody - BSA Free [NBP1-90299]

Staining of human cell line U-251 MG shows localization to nucleoplasm.
Immunohistochemistry-Frozen: gamma Sarcoglycan Antibody [NBP1-90299]

Immunohistochemistry-Frozen: gamma Sarcoglycan Antibody [NBP1-90299]

Immunohistochemistry-Frozen: gamma Sarcoglycan Antibody [NBP1-90299] - Human skeletal muscle tissue. IHC-Fr image submitted by a verified customer review.
gamma Sarcoglycan Antibody - BSA Free Immunohistochemistry: gamma Sarcoglycan Antibody - BSA Free [NBP1-90299]

Immunohistochemistry: gamma Sarcoglycan Antibody - BSA Free [NBP1-90299]

Analysis in human heart muscle and liver tissues using NBP1-90299 antibody. Corresponding SGCG RNA-seq data are presented for the same tissues.
gamma Sarcoglycan Antibody - BSA Free Immunohistochemistry: gamma Sarcoglycan Antibody - BSA Free [NBP1-90299]

Immunohistochemistry: gamma Sarcoglycan Antibody - BSA Free [NBP1-90299]

Staining of human heart muscle shows strong membranous positivity in cardiomyocytes.
gamma Sarcoglycan Antibody - BSA Free Immunohistochemistry: gamma Sarcoglycan Antibody - BSA Free [NBP1-90299]

Immunohistochemistry: gamma Sarcoglycan Antibody - BSA Free [NBP1-90299]

Staining of human liver shows no positivity in hepatocytes as expected.
gamma Sarcoglycan Antibody - BSA Free Immunohistochemistry: gamma Sarcoglycan Antibody - BSA Free [NBP1-90299]

Immunohistochemistry: gamma Sarcoglycan Antibody - BSA Free [NBP1-90299]

Staining of human skeletal muscle shows strong membranous positivity in myocytes.
gamma Sarcoglycan Antibody - BSA Free Immunohistochemistry: gamma Sarcoglycan Antibody - BSA Free [NBP1-90299]

Immunohistochemistry: gamma Sarcoglycan Antibody - BSA Free [NBP1-90299]

Staining of human prostate shows no positivity in glandular cells as expected.
gamma Sarcoglycan Antibody - BSA Free Western Blot: gamma Sarcoglycan Antibody - BSA Free [NBP1-90299]

Western Blot: gamma Sarcoglycan Antibody - BSA Free [NBP1-90299]

Analysis in control (vector only transfected HEK293T lysate) and SGCG over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).
gamma Sarcoglycan Antibody - BSA Free

Immunohistochemistry: gamma Sarcoglycan Antibody - BSA Free [NBP1-90299] -

Immunohistochemistry using antibodies against Dystrophin, Sarcoglycan-alpha, Sarcoglycan-beta, Sarcoglycan-gamma and Dysferlin showing normal expression in myofibres (a: Dystrophin x 20X, b: Sarcoglycan-alpha x 40X, c: Sarcoglycan-beta x 20X, d: Sarcoglycan-gamma x 40X, e: Dysferlin x 40X) Image collected and cropped by CiteAb from the following open publication (https://ojrd.biomedcentral.com/articles/10.1186/s13023-024-03158-1), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for gamma Sarcoglycan Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Frozen

Validated from a verified customer review.

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 1 review rated 5 using NBP1-90299 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: gamma-Sarcoglycan/SGCG

gamma Sarcoglycan encodes gamma-sarcoglycan, one of several sarcolemmal transmembrane glycoproteins that interact with dystrophin. The dystrophin-glycoprotein complex (DGC) spans the sarcolemma and is comprised of dystrophin, syntrophin, alpha- and beta-dystroglycans and sarcoglycans. The DGC provides a structural link between the subsarcolemmal cytoskeleton and the extracellular matrix of muscle cells. Defects in the encoded protein can lead to early onset autosomal recessive muscular dystrophy, in particular limb-girdle muscular dystrophy, type 2C (LGMD2C). [provided by RefSeq]

Long Name

Sarcoglycan, Gamma (35kDa Dystrophin-Associated Glycoprotein)

Alternate Names

DAGA4, DMDA, DMDA1, LGMD2C, LGMDR5, MGC130048, SCARMD2, SCG3

Entrez Gene IDs

6445 (Human)

Gene Symbol

SGCG

UniProt

Q13326 (Human)

Additional gamma-Sarcoglycan/SGCG Products

Product Documents for gamma Sarcoglycan Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for gamma Sarcoglycan Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Proteoglycans

Citations for gamma Sarcoglycan Antibody - BSA Free

Customer Reviews for gamma Sarcoglycan Antibody - BSA Free (1)

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  • gamma Sarcoglycan Antibody
    Name: Margherita verardo
    Application: Immunohistochemistry-Frozen
    Sample Tested: Skeletal muscle tissue
    Species: Human
    Verified Customer | Posted 10/08/2019
    gamma Sarcoglycan Antibody - BSA Free NBP1-90299

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