gamma Tubulin Antibody (TU-30) - BSA Free

Novus Biologicals | Catalog # NB500-574

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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Chicken, Plant, Protozoa

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # TU-30

Format

BSA Free
View all gamma Tubulin Primary Antibodies »

Product Specifications

Immunogen

C-terminal peptide of gamma-tubulin counjugated to KLH.

Epitope

The epitope was located in the amino acid sequence TRPDYI (aa439-444 in human), which is present on human gamma-tubulin 1 but not on human gamma-tubulin 2.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Marker

Centrosome Marker

Specificity

The antibody TU-30 recognizes C-terminal peptide sequence EYHAATRPDYISWGTQ (aa 434-449) of gamma-tubulin, a 48 kDa structural constituent of cytoskeleton and microtubule organizing center (MTOC).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for gamma Tubulin Antibody (TU-30) - BSA Free

Western Blot: gamma Tubulin Antibody (TU-30)BSA Free [NB500-574]

Western Blot: gamma Tubulin Antibody (TU-30)BSA Free [NB500-574]

Western Blot: gamma Tubulin Antibody (TU-30) [NB500-574] - Western blotting analysis of differential reactivity of monoclonal antibodies to gamma-tubulin with human gamma-tubulin isotypes. (A) Immunoblots of total cell lysates from SH-SY5Y cells, expressing TagRFP-tagged human gamma-tubulin 1 (gamma-Tb1) or gamma-tubulin 2 (gamma-Tb2), probed with Abs to gamma-tubulin (TU-30, TU-32), TagRFP (RFP) and GAPDH. In control samples, only secondary anti-mouse Ab was applied. (B) Immunoblots of immobilized GST-tagged human C-terminal regions (a.a. 362-451) of gamma-Tb1 or gamma-Tb2 probed with Abs to gamma-tubulin (TU-30, TU-32) and GST. In control samples, only secondary anti-mouse Ab was applied.
Immunocytochemistry/ Immunofluorescence: gamma Tubulin Antibody (TU-30) - BSA Free [NB500-574]

Immunocytochemistry/ Immunofluorescence: gamma Tubulin Antibody (TU-30) - BSA Free [NB500-574]

Immunocytochemistry/Immunofluorescence: gamma Tubulin Antibody (TU-30) [NB500-574] - Staining of HeLa cells using anti-gamma Tubulin antibody (green). Nuclei were stained with DAPI (blue). Image from verified customer review.
Immunohistochemistry-Paraffin: gamma Tubulin Antibody (TU-30) - BSA Free [NB500-574]

Immunohistochemistry-Paraffin: gamma Tubulin Antibody (TU-30) - BSA Free [NB500-574]

Immunohistochemistry-Paraffin: gamma Tubulin Antibody (TU-30) - BSA Free [NB500-574] - FFPE Glioblastoma PDX is stained with mouse-gamma tubulin antibody (NB500-574) (red) for centrosomes and rabbit Arl13B (green) for primary cilia. Gamma Tubulin antibody is diluted 1:25, incubated overnight at 4C. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: gamma Tubulin Antibody (TU-30) - BSA Free [NB500-574]

Immunocytochemistry/ Immunofluorescence: gamma Tubulin Antibody (TU-30) - BSA Free [NB500-574]

Immunocytochemistry/Immunofluorescence: gamma Tubulin Antibody (TU-30) [NB500-574] - Staining of P19X1 mouse embryonal carcinoma cell line using anti-gamma-tubulin (TU-30) (detection by secondary antibody Goat anti-mouse Cy3). Nuclei were stained with DAPI (blue).
Immunocytochemistry/ Immunofluorescence: gamma Tubulin Antibody (TU-30) - BSA Free [NB500-574]

Immunocytochemistry/ Immunofluorescence: gamma Tubulin Antibody (TU-30) - BSA Free [NB500-574]

Immunocytochemistry/Immunofluorescence: gamma Tubulin Antibody (TU-30) [NB500-574] - Staining of mouse fibroblasts using anti-gamma-tubulin (TU-30; direct conjugate with Dyomics 547, red). Nuclei were stained with DAPI (blue).
Immunocytochemistry/ Immunofluorescence: gamma Tubulin Antibody (TU-30) - BSA Free [NB500-574]

Immunocytochemistry/ Immunofluorescence: gamma Tubulin Antibody (TU-30) - BSA Free [NB500-574]

Immunocytochemistry/Immunofluorescence: gamma Tubulin Antibody (TU-30) [NB500-574] - Staining of microtubular networks in 3T3 mouse fibroblasts. 3A - metaphase; 3B - anaphase; 3C - telophase Gamma-tubulin (red) stained with anti-gamma-tubulin (TU-30), alpha-tubulin (green) with polyclonal anti-alpha-tubulin antibody and nuclei with DAPI (blue).
gamma Tubulin Antibody (TU-30) - BSA Free Western Blot: gamma Tubulin Antibody (TU-30) - BSA Free [NB500-574]

Western Blot: gamma Tubulin Antibody (TU-30) - BSA Free [NB500-574]

Analysis of human gamma-tubulin using mouse monoclonal antibody TU-30 on lysates of various cell lines under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-gamma-tubulin monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for gamma-tubulin at approximately 46 kDa.

Applications for gamma Tubulin Antibody (TU-30) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Western Blot

1:1000
Application Notes
Immunocytochemistry - Staining technique: (a) Fix cells for 10 min in methanol at -20oC and for 6 min in acetone at -20oC; (b) Fix cells directly in methanol for 10 min at -20oC or in acetone for 10 min at -20oC. Incubation: 45 min in room temperature Application note: The antibody TU-30 stains only fixed cells. gamma Tubulin Antibody (TU-30) - BSA Free validated for Immunohistochemistry-Paraffin from a verified customer review.

Reviewed Applications

Read 2 reviews rated 4.5 using NB500-574 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: gamma Tubulin

The gamma-tubulin (TUBG1; relative molecular weight about 48 kDa) is a minor member of tubulin family (less that 0.01% of tubulin dimer). The gamma-tubulin ring structures, however, serve to provide structural primer for initiation of microtubular nucleation and growth, thereby being crutial for microtubule-based cellular processes, above all for mitotic spindle formation. In animal cells, a center of microtubule organization is the centrosome composed of a pair of cylindrical centrioles surrounded by fibrous pericentriolar material containing gamma-tubulin. Formation of the mitotic spindle is preceded by duplication of centrosome during S phase. Before mitosis, both centrosomes increase their microtubule nucleation capacity and form two microtuble asters that are pushed apart from each other by the forces of motor proteins associated at the microtubule surface.

Long Name

Tubulin gamma-1 chain

Alternate Names

Gamma-1-tubulin, GCP-1, TUBG, TUBG1

Gene Symbol

TUBG1

Additional gamma Tubulin Products

Product Documents for gamma Tubulin Antibody (TU-30) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for gamma Tubulin Antibody (TU-30) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for gamma Tubulin Antibody (TU-30) - BSA Free

Customer Reviews for gamma Tubulin Antibody (TU-30) - BSA Free (2)

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Customer Images

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  • gamma Tubulin Antibody (TU-30) - BSA Free
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: human glioblastoma
    Species: Human
    Verified Customer | Posted 09/06/2022
    F-IHC. FFPE Glioblastoma PDX is stained with mouse-gamma tubulin antibody (NB500-574) (red) for centrosomes and rabbit Arl13B (green) for primary cilia. Gamma Tubulin antibody is diluted 1:25, incubated overnight at 4C.
    gamma Tubulin Antibody (TU-30) - BSA Free NB500-574
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: HeLa cells
    Species: Human
    Verified Customer | Posted 08/16/2016
    Hela Cells with gamma Tubulin and DAPI
    gamma Tubulin Antibody (TU-30) - BSA Free NB500-574

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