GATA-1 Antibody

Novus Biologicals | Catalog # NB100-2443

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Canine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all GATA-1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-DAEAYRHSPVFQ corresponding to internal region according to NP_002040.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for GATA-1 Antibody

Western Blot: GATA-1 Antibody [NB100-2443]

Western Blot: GATA-1 Antibody [NB100-2443]

Western Blot: GATA-1 Antibody [NB100-2443] - Staining of K562 nuclear cell lysate (A) and negative control Human Hippocampus (B) lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: GATA-1 Antibody [NB100-2443]

Immunocytochemistry/ Immunofluorescence: GATA-1 Antibody [NB100-2443]

Immunocytochemistry/Immunofluorescence: GATA-1 Antibody [NB100-2443] - Analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Flow Cytometry: GATA-1 Antibody [NB100-2443]

Flow Cytometry: GATA-1 Antibody [NB100-2443]

Flow Cytometry: GATA-1 Antibody [NB100-2443] - Analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunocytochemistry/ Immunofluorescence: GATA-1 Antibody [NB100-2443]

Immunocytochemistry/ Immunofluorescence: GATA-1 Antibody [NB100-2443]

Immunocytochemistry/Immunofluorescence: GATA-1 Antibody [NB100-2443] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Applications for GATA-1 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:16000

Western Blot

0.3 - 1 ug/mL
Application Notes
Western blot: Approx 50-55 kDa band observed in nuclear lysates of cell line K562 calculated MW of 42.8 kDa according to NP_002040.1).

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GATA-1

GATA1 (Globin transcription factor 1) is a Cys2/Cys2 zinc finger DNA binding protein that is expressed primarily in erythroid, megakaryocytic, mast cells and eosinophilic cells. It belongs to the GATA family of transcription factors. GATA1 is a transcriptional activator which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence [AT]GATA[AG] within regulatory regions of globin genes and of other genes expressed in erythroid cells. The protein also plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia. Acquired somatic mutations in GATA1 occur in virtually all children with Down's Syndrome, congenital transient myeloproliferative syndrome (TMD) and acute megakaryocytic leukemia.

Alternate Names

GATA1

Entrez Gene IDs

2623 (Human)

Gene Symbol

GATA1

UniProt

NP_002040.1 (Human)

Additional GATA-1 Products

Product Documents for GATA-1 Antibody

Certificate of Analysis

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Product Specific Notices for GATA-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GATA-1 Antibody

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Protocols

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