GATA-1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84793

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GATA-1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EGLDAAASSTAPSTATAAAAALAYYRDAEAYRHSPVFQVYPLLNCMEGIPGGSPYAGWAYGKTGLYPASTVCPTREDSPPQAVEDLDGKGSTSFLETLKTERLSPDLLTLGPALP

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (81%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GATA-1 Antibody - BSA Free

GATA-1 Antibody - BSA Free Immunohistochemistry-Paraffin: GATA-1 Antibody - BSA Free [NBP1-84793]

Immunohistochemistry-Paraffin: GATA-1 Antibody - BSA Free [NBP1-84793]

Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
GATA-1 Antibody - BSA Free Immunohistochemistry-Paraffin: GATA-1 Antibody - BSA Free [NBP1-84793]

Immunohistochemistry-Paraffin: GATA-1 Antibody - BSA Free [NBP1-84793]

Analysis in human bone marrow and pancreas tissues using HPA000232 antibody. Corresponding GATA1 RNA-seq data are presented for the same tissues.
GATA-1 Antibody - BSA Free Immunohistochemistry-Paraffin: GATA-1 Antibody - BSA Free [NBP1-84793]

Immunohistochemistry-Paraffin: GATA-1 Antibody - BSA Free [NBP1-84793]

Staining of human liver shows no positivity in hepatocytes as expected.
GATA-1 Antibody - BSA Free Western Blot: GATA-1 Antibody - BSA Free [NBP1-84793]

Western Blot: GATA-1 Antibody - BSA Free [NBP1-84793]

Analysis in control (vector only transfected HEK293T lysate) and GATA1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400758).
GATA-1 Antibody - BSA Free Immunohistochemistry-Paraffin: GATA-1 Antibody - BSA Free [NBP1-84793]

Immunohistochemistry-Paraffin: GATA-1 Antibody - BSA Free [NBP1-84793]

Staining of human skeletal muscle shows no positivity in myocytes as expected.
GATA-1 Antibody - BSA Free Immunohistochemistry-Paraffin: GATA-1 Antibody - BSA Free [NBP1-84793]

Immunohistochemistry-Paraffin: GATA-1 Antibody - BSA Free [NBP1-84793]

Staining of human bone marrow shows strong nuclear positivity in hematopoietic cells.
GATA-1 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: GATA-1 Antibody - BSA Free [NBP1-84793]

Chromatin Immunoprecipitation-exo-Seq: GATA-1 Antibody - BSA Free [NBP1-84793]

ChIP-Exo-Seq composite graph for Anti-GATA1 (NBP1-84793) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for GATA-1 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GATA-1

GATA1 (Globin transcription factor 1) is a Cys2/Cys2 zinc finger DNA binding protein that is expressed primarily in erythroid, megakaryocytic, mast cells and eosinophilic cells. It belongs to the GATA family of transcription factors. GATA1 is a transcriptional activator which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence [AT]GATA[AG] within regulatory regions of globin genes and of other genes expressed in erythroid cells. The protein also plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia. Acquired somatic mutations in GATA1 occur in virtually all children with Down's Syndrome, congenital transient myeloproliferative syndrome (TMD) and acute megakaryocytic leukemia.

Alternate Names

GATA1

Gene Symbol

GATA1

Additional GATA-1 Products

Product Documents for GATA-1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for GATA-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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