GATM Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89211

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (91%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GATM Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LGRTLTGWVQRTFQSTQAATASSRNSCAADDKATEPLPKDCPVSSYNEWDPLEEVIVGRAENACVPPFTIEVKANTYEKYWPFYQKQGGHYFPKDHLKKAVAEIEEMCNILKTEGVTVRRPDPIDWSLKYKTPD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GATM Antibody - BSA Free

Immunohistochemistry-Paraffin: GATM Antibody [NBP1-89211]

Immunohistochemistry-Paraffin: GATM Antibody [NBP1-89211]

Immunohistochemistry-Paraffin: GATM Antibody [NBP1-89211] - Staining in human kidney and skeletal muscle tissues. Corresponding GATM RNA-seq data are presented for the same tissues.
Western Blot: GATM Antibody [NBP1-89211]

Western Blot: GATM Antibody [NBP1-89211]

Western Blot: GATM Antibody [NBP1-89211] - Analysis in human kidney tissue.
Immunocytochemistry/ Immunofluorescence: GATM Antibody [NBP1-89211]

Immunocytochemistry/ Immunofluorescence: GATM Antibody [NBP1-89211]

Immunocytochemistry/Immunofluorescence: GATM Antibody [NBP1-89211] - Staining of human cell line RH-30 shows localization to mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: GATM Antibody [NBP1-89211]

Immunohistochemistry-Paraffin: GATM Antibody [NBP1-89211]

Immunohistochemistry-Paraffin: GATM Antibody [NBP1-89211] - Staining of human duodenum shows moderate to strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: GATM Antibody [NBP1-89211]

Immunohistochemistry-Paraffin: GATM Antibody [NBP1-89211]

Immunohistochemistry-Paraffin: GATM Antibody [NBP1-89211] - Staining of human kidney shows moderate to strong cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: GATM Antibody [NBP1-89211]

Immunohistochemistry-Paraffin: GATM Antibody [NBP1-89211]

Immunohistochemistry-Paraffin: GATM Antibody [NBP1-89211] - Staining of human liver shows moderate to strong cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: GATM Antibody [NBP1-89211]

Immunohistochemistry-Paraffin: GATM Antibody [NBP1-89211]

Immunohistochemistry-Paraffin: GATM Antibody [NBP1-89211] - Staining of human skeletal muscle shows no cytoplasmic positivity in myocytes as expected.

Applications for GATM Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GATM

GATM encodes a mitochondrial enzyme that belongs to the amidinotransferase family. This enzyme is involved in creatine biosynthesis, whereby it catalyzes the transfer of a guanido group from L-arginine to glycine, resulting in guanidinoacetic acid, the immediate precursor of creatine. Mutations in this gene cause arginine:glycine amidinotransferase deficiency, an inborn error of creatine synthesis characterized by mental retardation, language impairment, and behavioral disorders.

Alternate Names

AGATtransamidinase, AT, EC 2.1.4, EC 2.1.4.1, glycine amidinotransferase (L-arginine:glycine amidinotransferase), glycine amidinotransferase, mitochondrial, L-arginine:glycine amidinotransferase, Transamidinase

Gene Symbol

GATM

Additional GATM Products

Product Documents for GATM Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for GATM Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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