Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1B8

Format

Azide and BSA Free
View all GBAS Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human GBAS(NP_001474) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

33.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GBAS Antibody (OTI1B8) - Azide and BSA Free

Western Blot: GBAS Antibody (OTI1B8)Azide and BSA Free [NBP2-70804]

Western Blot: GBAS Antibody (OTI1B8)Azide and BSA Free [NBP2-70804]

Western Blot: GBAS Antibody (OTI1B8) - Azide and BSA Free [NBP2-70804] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBAS.
Immunocytochemistry/ Immunofluorescence: GBAS Antibody (OTI1B8) - Azide and BSA Free [NBP2-70804]

Immunocytochemistry/ Immunofluorescence: GBAS Antibody (OTI1B8) - Azide and BSA Free [NBP2-70804]

Immunocytochemistry/Immunofluorescence: GBAS Antibody (OTI1B8) - Azide and BSA Free [NBP2-70804] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY GBAS.
Immunohistochemistry: GBAS Antibody (OTI1B8) - Azide and BSA Free [NBP2-70804]

Immunohistochemistry: GBAS Antibody (OTI1B8) - Azide and BSA Free [NBP2-70804]

Immunohistochemistry: GBAS Antibody (OTI1B8) - Azide and BSA Free [NBP2-70804] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: GBAS Antibody (OTI1B8)Azide and BSA Free [NBP2-70804]

Western Blot: GBAS Antibody (OTI1B8)Azide and BSA Free [NBP2-70804]

Western Blot: GBAS Antibody (OTI1B8) - Azide and BSA Free [NBP2-70804] - Analysis of extracts (10ug) from 8 Human tissue (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).
Western Blot: GBAS Antibody (OTI1B8)Azide and BSA Free [NBP2-70804]

Western Blot: GBAS Antibody (OTI1B8)Azide and BSA Free [NBP2-70804]

Western Blot: GBAS Antibody (OTI1B8) - Azide and BSA Free [NBP2-70804] - Analysis of extracts (35ug) from 9 different cell lines by using g GBAS monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunocytochemistry/ Immunofluorescence: GBAS Antibody (OTI1B8) - Azide and BSA Free [NBP2-70804]

Immunocytochemistry/ Immunofluorescence: GBAS Antibody (OTI1B8) - Azide and BSA Free [NBP2-70804]

Immunocytochemistry/Immunofluorescence: GBAS Antibody (OTI1B8) - Azide and BSA Free [NBP2-70804] - Analysis of HeLa.
Immunohistochemistry: GBAS Antibody (OTI1B8) - Azide and BSA Free [NBP2-70804]

Immunohistochemistry: GBAS Antibody (OTI1B8) - Azide and BSA Free [NBP2-70804]

Immunohistochemistry: GBAS Antibody (OTI1B8) - Azide and BSA Free [NBP2-70804] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: GBAS Antibody (OTI1B8) - Azide and BSA Free [NBP2-70804]

Immunohistochemistry: GBAS Antibody (OTI1B8) - Azide and BSA Free [NBP2-70804]

Immunohistochemistry: GBAS Antibody (OTI1B8) - Azide and BSA Free [NBP2-70804] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: GBAS Antibody (OTI1B8) - Azide and BSA Free [NBP2-70804]

Immunohistochemistry: GBAS Antibody (OTI1B8) - Azide and BSA Free [NBP2-70804]

Immunohistochemistry: GBAS Antibody (OTI1B8) - Azide and BSA Free [NBP2-70804] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Applications for GBAS Antibody (OTI1B8) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunohistochemistry

1:150

Western Blot

1:200-1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GBAS

Chromosomal region 7p12, which contains GBAS, is amplified in approximately 40% of glioblastomas, the most common andmalignant form of central nervous system tumor.The predicted 286-amino acid protein contains a signal peptide, atransmembrane domain, and 2 tyrosine phosphorylation sites. The GBAS transcript is expressed most abundantly in heartand skeletal muscle. GBAS protein might be involved in vesicular transport. (provided by RefSeq)

Alternate Names

glioblastoma amplified sequence, Glioblastoma-amplified sequence, NIPSNAP2NipSnap2,4-nitrophenylphosphatase domain and non-neuronal SNAP25-like 2, protein NipSnap homolog 2

Gene Symbol

NIPSNAP2

Additional GBAS Products

Product Documents for GBAS Antibody (OTI1B8) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for GBAS Antibody (OTI1B8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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