GBP2 Antibody (OTI5C8)

Novus Biologicals | Catalog # NBP1-47768

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI5C8
View all GBP2 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human GBP2 (NP_004111) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GBP2 Antibody (OTI5C8)

Western Blot: GBP2 Antibody (OTI5C8) [NBP1-47768]

Western Blot: GBP2 Antibody (OTI5C8) [NBP1-47768]

Western Blot: GBP2 Antibody (OTI5C8) [NBP1-47768] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GBP2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
Immunocytochemistry/ Immunofluorescence: GBP2 Antibody (OTI5C8) [NBP1-47768]

Immunocytochemistry/ Immunofluorescence: GBP2 Antibody (OTI5C8) [NBP1-47768]

Immunocytochemistry/Immunofluorescence: GBP2 Antibody (OTI5C8) [NBP1-47768] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY GBP2.
Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768]

Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768]

Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768] - Staining of paraffin-embedded Human colon tissue within the normal limits using anti-GBP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min)
Flow Cytometry: GBP2 Antibody (OTI5C8) [NBP1-47768]

Flow Cytometry: GBP2 Antibody (OTI5C8) [NBP1-47768]

Flow Cytometry: GBP2 Antibody (OTI5C8) [NBP1-47768] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GBP2 antibody, and then analyzed by flow cytometry.
Western Blot: GBP2 Antibody (OTI5C8) [NBP1-47768]

Western Blot: GBP2 Antibody (OTI5C8) [NBP1-47768]

Western Blot: GBP2 Antibody (OTI5C8) [NBP1-47768] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GBP2.
Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768]

Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768]

Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-GBP2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768]

Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768]

Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-GBP2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768]

Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768]

Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-GBP2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768]

Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768]

Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-GBP2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768]

Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768]

Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768] - Staining of paraffin-embedded Human colon tissue using anti-GBP2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768]

Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768]

Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768] - Staining of paraffin-embedded Human Kidney tissue using anti-GBP2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768]

Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768]

Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768] - Staining of paraffin-embedded Human Ovary tissue using anti-GBP2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768]

Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768]

Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768] - Staining of paraffin-embedded Human prostate tissue using anti-GBP2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768]

Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768]

Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768] - Staining of paraffin-embedded Human thyroid tissue using anti-GBP2 mouse monoclonal antibody.
Immunohistochemistry: GBP2 Antibody (OTI5C8) [NBP1-47768]

Immunohistochemistry: GBP2 Antibody (OTI5C8) [NBP1-47768]

Immunohistochemistry: GBP2 Antibody (OTI5C8) [NBP1-47768] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-GBP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min

Applications for GBP2 Antibody (OTI5C8)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:2000

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GBP2

Interferons are cytokines that have antiviral effects and inhibit tumor cell proliferation. They induce a large number of genes in their target cells, including those coding for the guanylate-binding proteins (GBPs). GBPs are characterized by their ability to specifically bind guanine nucleotides (GMP, GDP, and GTP). The protein encoded by this gene is a GTPase that converts GTP to GDP and GMP.

Alternate Names

GBP-2, GTP-binding protein 2, Guanine nucleotide-binding protein 2, guanylate binding protein 2, interferon-inducible, huGBP-2, interferon-induced guanylate-binding protein 2

Entrez Gene IDs

2634 (Human)

Gene Symbol

GBP2

UniProt

P32456 (Human)

Additional GBP2 Products

Product Documents for GBP2 Antibody (OTI5C8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for GBP2 Antibody (OTI5C8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GBP2 Antibody (OTI5C8)

Customer Reviews for GBP2 Antibody (OTI5C8)

There are currently no reviews for this product. Be the first to review GBP2 Antibody (OTI5C8) and earn rewards!

Have you used GBP2 Antibody (OTI5C8)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies