GCLC Antibody (3H1) - Azide and BSA Free

Novus Biologicals | Catalog # H00002729-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3H1

Format

Azide and BSA Free
View all GCLC Primary Antibodies »

Product Specifications

Immunogen

GCLC (NP_001489, 528 a.a. ~ 637 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EGVFPGLIPILNSYLENMEVDVDTRCSILNYLKLIKKRASGELMTVARWMREFIANHPDYKQDSVITDEMNYSLILKCNQIANELCECPELLGSAFRKVKYSGSKTDSSN

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

GCLC - glutamate-cysteine ligase, catalytic subunit

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for GCLC Antibody (3H1) - Azide and BSA Free

Western Blot: GCLC Antibody (3H1) [H00002729-M01]

Western Blot: GCLC Antibody (3H1) [H00002729-M01]

Western Blot: GCLC Antibody (3H1) [H00002729-M01] - Analysis of GCLC expression in transfected 293T cell line by GCLC monoclonal antibody (M01), clone 3H1.Lane 1: GCLC transfected lysate(72.8 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: GCLC Antibody (3H1) [H00002729-M01]

Immunocytochemistry/ Immunofluorescence: GCLC Antibody (3H1) [H00002729-M01]

Immunocytochemistry/Immunofluorescence: GCLC Antibody (3H1) [H00002729-M01] - Analysis of monoclonal antibody to GCLC on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: GCLC Antibody (3H1) [H00002729-M01]

Western Blot: GCLC Antibody (3H1) [H00002729-M01]

Western Blot: GCLC Antibody (3H1) [H00002729-M01] - GCLC monoclonal antibody (M01), clone 3H1. Analysis of GCLC expression in PC-12.
Western Blot: GCLC Antibody (3H1) [H00002729-M01]

Western Blot: GCLC Antibody (3H1) [H00002729-M01]

Western Blot: GCLC Antibody (3H1) [H00002729-M01] - GCLC monoclonal antibody (M01), clone 3H1 Analysis of GCLC expression in A-431.
Western Blot: GCLC Antibody (3H1) [H00002729-M01]

Western Blot: GCLC Antibody (3H1) [H00002729-M01]

Western Blot: GCLC Antibody (3H1) [H00002729-M01] - GCLC monoclonal antibody (M01), clone 3H1. Analysis of GCLC expression in NIH/3T3.
ELISA: GCLC Antibody (3H1) [H00002729-M01]

ELISA: GCLC Antibody (3H1) [H00002729-M01]

ELISA: GCLC Antibody (3H1) [H00002729-M01] - Detection limit for recombinant GST tagged GCLC is approximately 0.03ng/ml as a capture antibody.

Applications for GCLC Antibody (3H1) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GCLC

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene encoding the catalytic subunit encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kDa and maps to chromosome 6. The regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.

Long Name

Glutamate--cysteine ligase catalytic subunit

Alternate Names

EC 6.3.2.2, Gamma-ECS, GCS heavy chain, GLCL, GLCLC

Entrez Gene IDs

2729 (Human)

Gene Symbol

GCLC

OMIM

230450 (Human)

UniProt

NP_001489 (Human)

Additional GCLC Products

Product Documents for GCLC Antibody (3H1) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for GCLC Antibody (3H1) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GCLC Antibody (3H1) - Azide and BSA Free

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Protocols

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FAQs

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