GCNF/NR6A1 Antibody (4G8) - BSA Free

Novus Biologicals | Catalog # NBP2-37467

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4G8

Format

BSA Free
View all GCNF/NR6A1 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human GCNF/NR6A1 (AA: 65-118) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

54.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GCNF/NR6A1 Antibody (4G8) - BSA Free

Western Blot: GCNF/NR6A1 Antibody (4G8)BSA Free [NBP2-37467]

Western Blot: GCNF/NR6A1 Antibody (4G8)BSA Free [NBP2-37467]

Western Blot: GCNF/NR6A1 Antibody (4G8) [NBP2-37467] - Western blot analysis using NR6A1 mouse mAb against K562 (1), NTERA-2 (2), HEK293 (3), HUVE-12 (4), and HeLa (5) cell lysate.
Immunocytochemistry/ Immunofluorescence: GCNF/NR6A1 Antibody (4G8) - BSA Free [NBP2-37467]

Immunocytochemistry/ Immunofluorescence: GCNF/NR6A1 Antibody (4G8) - BSA Free [NBP2-37467]

Immunocytochemistry/Immunofluorescence: GCNF/NR6A1 Antibody (4G8) [NBP2-37467] - Immunofluorescence analysis of HepG2 cells using NR6A1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry: GCNF/NR6A1 Antibody (4G8) - BSA Free [NBP2-37467]

Immunohistochemistry: GCNF/NR6A1 Antibody (4G8) - BSA Free [NBP2-37467]

Immunohistochemistry: GCNF/NR6A1 Antibody (4G8) [NBP2-37467] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using NR6A1 mouse mAb with DAB staining.
Flow Cytometry: GCNF/NR6A1 Antibody (4G8) - BSA Free [NBP2-37467]

Flow Cytometry: GCNF/NR6A1 Antibody (4G8) - BSA Free [NBP2-37467]

Flow Cytometry: GCNF/NR6A1 Antibody (4G8) [NBP2-37467] - Flow cytometric analysis of K562 cells using NR6A1 mouse mAb (green) and negative control (red).
Western Blot: GCNF/NR6A1 Antibody (4G8)BSA Free [NBP2-37467]

Western Blot: GCNF/NR6A1 Antibody (4G8)BSA Free [NBP2-37467]

Western Blot: GCNF/NR6A1 Antibody (4G8) [NBP2-37467] - Western blot analysis using NR6A1 mAb against human NR6A1 recombinant protein. (Expected MW is 32.4 kDa)
Western Blot: GCNF/NR6A1 Antibody (4G8)BSA Free [NBP2-37467]

Western Blot: GCNF/NR6A1 Antibody (4G8)BSA Free [NBP2-37467]

Western Blot: GCNF/NR6A1 Antibody (4G8) [NBP2-37467] - Western blot analysis using NR6A1 mAb against HEK293 (1) and NR6A1 (AA: 65-118)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry: GCNF/NR6A1 Antibody (4G8) - BSA Free [NBP2-37467]

Immunohistochemistry: GCNF/NR6A1 Antibody (4G8) - BSA Free [NBP2-37467]

Immunohistochemistry: GCNF/NR6A1 Antibody (4G8) [NBP2-37467] - Immunohistochemical analysis of paraffin-embedded cerebellum tissues using NR6A1 mouse mAb with DAB staining.
ELISA: GCNF/NR6A1 Antibody (4G8) - BSA Free [NBP2-37467]

ELISA: GCNF/NR6A1 Antibody (4G8) - BSA Free [NBP2-37467]

ELISA: GCNF/NR6A1 Antibody (4G8) [NBP2-37467] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for GCNF/NR6A1 Antibody (4G8) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:400

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GCNF/NR6A1

GCNF encodes an orphan nuclear receptor which is a member of the nuclear hormone receptor family. Its expression pattern suggests that it may be involved in neurogenesis and germ cell development. The protein can homodimerize and bind DNA, but in vivo targets have not been identified. The gene expresses at least alternatively spliced transcript variants.

Long Name

Germ Cell Nuclear Factor

Alternate Names

NR6A1, RTR

Gene Symbol

NR6A1

Additional GCNF/NR6A1 Products

Product Documents for GCNF/NR6A1 Antibody (4G8) - BSA Free

Certificate of Analysis

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Product Specific Notices for GCNF/NR6A1 Antibody (4G8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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