GCP4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-90313

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GCP4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ILFVGESVQMFENQNVNLTRKGSILKNQEDTFAAELHRLKQQPLFSLVDFEQVVDRIRSTVAEHLWKLMVEESDLLGQLKIIKDFYLLGRGELFQAFIDTAQHMLKTPPTA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GCP4 Antibody - BSA Free

GCP4 Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: GCP4 Antibody - BSA Free [NBP1-90313]

Immunocytochemistry/ Immunofluorescence: GCP4 Antibody - BSA Free [NBP1-90313]

Staining of human cell line A-431 shows positivity in centrosome.
GCP4 Antibody - BSA Free Immunohistochemistry: GCP4 Antibody - BSA Free [NBP1-90313]

Immunohistochemistry: GCP4 Antibody - BSA Free [NBP1-90313]

Staining of human duodenum shows moderate granular cytoplasmic positivity in glandular cells.
GCP4 Antibody - BSA Free Western Blot: GCP4 Antibody - BSA Free [NBP1-90313]

Western Blot: GCP4 Antibody - BSA Free [NBP1-90313]

Analysis in control (vector only transfected HEK293T lysate) and TUBGCP4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).

Applications for GCP4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GCP4

Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome

Alternate Names

76PhGCP4, FLJ14797, gamma tubulin ring complex protein (76p gene), Gamma-ring complex protein 76 kDa, gamma-tubulin complex component 4, GCP-4, GCP4hGrip76, h76p, tubulin, gamma complex associated protein 4

Gene Symbol

TUBGCP4

Additional GCP4 Products

Product Documents for GCP4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for GCP4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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