GDNF Antibody (11D2) - BSA Free

Novus Biologicals | Catalog # NBP3-26556

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA, Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 11D2 expressed in HEK293

Format

BSA Free
View all GDNF Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human GDNF [UniProt P39905]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GDNF Antibody (11D2) - BSA Free

GDNF Antibody (11D2)

Immunoprecipitation: GDNF Antibody (11D2) [NBP3-26556] -

Immunoprecipitation: GDNF Antibody (11D2) [NBP3-26556] - Immunoprecipitating GDNF in Hela whole cell lysate.
Lane 1: Rabbit control IgG instead of NBP3-26556 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).
Lane 2: NBP3-26556 (3ug) + Hela whole cell lysate (500ug).
Lane 3: Hela whole cell lysate (20ug).
GDNF Antibody (11D2)

Immunohistochemistry: GDNF Antibody (11D2) [NBP3-26556] -

Immunohistochemistry: GDNF Antibody (11D2) [NBP3-26556] - Image of GDNF Antibody (11D2) diluted at 1:107.5 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
GDNF Antibody (11D2)

Immunohistochemistry: GDNF Antibody (11D2) [NBP3-26556] -

Immunohistochemistry: GDNF Antibody (11D2) [NBP3-26556] - Image of GDNF Antibody (11D2) diluted at 1:107.5 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
GDNF Antibody (11D2)

Western Blot: GDNF Antibody (11D2) [NBP3-26556] -

Western Blot: GDNF Antibody (11D2) [NBP3-26556] - Positive Western Blot detected in: HepG2 whole cell lysate, 293T whole cell lysate, SH-SY5Y whole cell lysate, PC3 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate, U251 whole cell lysate.
All lanes: GDNF Antibody at 1ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 24, 21, 26, 23, 19 KDa
Observed band size: 24 KDa
GDNF Antibody (11D2)

Flow Cytometry: GDNF Antibody (11D2) [NBP3-26556] -

Flow Cytometry: GDNF Antibody (11D2) [NBP3-26556] - Overlay histogram showing SH-SY5Y cells stained with GDNF Antibody (11D2) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

Applications for GDNF Antibody (11D2) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:200-1:1000

Western Blot

1:500-1:5000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: GDNF

GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was then shown to rescue these neurons in animal models of Parkinson's disease. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. ; FUNCTION: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. SUBUNIT: Homodimer; disulfide-linked. SUBCELLULAR LOCATION: Secreted protein. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. ; DISEASE: Defects in GDNF may be a cause of Hirschsprung disease (HSCR). In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. DISEASE: Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. SIMILARITY: Belongs to the TGF-beta family. GDNF subfamily.

Long Name

Glial Cell line-derived Growth Factor

Alternate Names

ATF, HFB1-GDNF, HGDNF, HSCR3

Gene Symbol

GDNF

Additional GDNF Products

Product Documents for GDNF Antibody (11D2) - BSA Free

Certificate of Analysis

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Product Specific Notices for GDNF Antibody (11D2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

GDNF Family

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Protocols

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